ABBA, ZUBAINATU and MOHAMAD FUZI, SITI FATIMAH ZAHARAH and HAZEL MONICA MATIAS-PERALTA, HAZEL MONICA MATIAS-PERALTA (2023) Identification of Carbohydrates Metabolic Related Enzymes for Lipid Production in Botryococcus sp., a Microalgae Isolated from Taman Negara Endau Rompin. Borneo Journal of Resource Science and Technology, 13 (1). pp. 42-53.
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Abstract
Botryococcus is a microalgal genus known for its ability to generate and accumulate substantial amounts of lipids via carbohydrate metabolism. This work determined the metabolic pathways and enzymes involved in carbohydrate metabolism leading to increased synthesis of fat in Botryococcus sp. Relevant intracellular and extracellular metabolites were extracted and quantified using chromatographic analysis. Enzymes involved in carbohydrate metabolism leading to lipid formation in Botryococcus sp. under natural conditions were also discovered by one-dimensional gel electrophoresis followed by proteomic mass spectrometry (LC-MS/MS) and database searching. Proximate analysis demonstrated 23.0% total carbohydrate, 16.0% protein and 61.0% lipid per milligram biomass dry weight of Botryococcus sp. The extracellular metabolites constitute mostly of cyclohydrocarbons, nitrogenated hydrocarbons, siloxanes, phenols, and phenol derivatives. A glycolytic enzyme “enolase,” which can create phosphoenolpyruvate and subsequently convert it into pyruvate, was found in this study. This study revealed that enolase provided an alternate pathway to export fixed carbon to the cytoplasm, hence providing a shorter route to lipid production than the normal process via the plastid leading to the manufacture of more lipids in the cells of Botryococcus sp. than other microalgae of the same group.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Botryococcus sp., carbohydrate metabolism, enolase, lipid, phosphoenolpyruvate |
| Subjects: | T Technology > TP Chemical technology > TP248.13-248.65 Biotechnology |
| Divisions: | Faculty of Applied Science and Technology > Department of Technology and Natural Resources |
| Depositing User: | Mr. Mohamad Zulkhibri Rahmad |
| Date Deposited: | 17 Oct 2023 07:22 |
| Last Modified: | 17 Oct 2023 07:22 |
| URI: | http://eprints.uthm.edu.my/id/eprint/10119 |
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