Microalgae scenedesmus sp. Bioreactor for removal of nutrients and organic contents from wet market wastewater

Jais, Noor Maisara (2019) Microalgae scenedesmus sp. Bioreactor for removal of nutrients and organic contents from wet market wastewater. Doctoral thesis, Universiti Tun Hussein Onn Malaysia.


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Wet market allows customers to get fresh foodstuff. However, its wet market wastewater (WMWW) which contains COD, BOD, TSS, NH3-N and P is commonly discharged directly into drainage without any treatment. Hence, the main aim of this research is to develop a prototype of Scenedesmus sp. bioreactor that can carry out phycoremediation process and ensure the parameters in WMWW can reach allowable standard. 16 types of samples based on four different initial concentration of Scenedesmus sp. (1×106 cell/mL, 10×106 cell/mL, 100×106 cell/mL, 1000×106 cell/mL) with presence or absence of agitator were optimized. The best growth rate and the highest reduction of nutrients and organic contents was chosen as optimum initial concentration of Scenedesmus sp. to be used in bioreactor. Scenedesmus sp. bioreactor was developed properly with four specific tanks and auto sensors for time and water level. The size is 15 cm (length) × 15 cm (width) × 30 cm (height) for each tank. Results showed that 100×106 cell/mL Scenedesmus sp. with agitated condition during optimization gave highest efficiency removal COD, BOD, NH3-N and P which are >91% with highest growth rate 0.667 μ/day and 2.71×109 cell/mL biomass production. The concentration of COD, BOD, NH3-N and P using Scenedesmus sp. bioreactor with (p<0.05) are pass sewage effluent Standard B. Reaction rate constant, k = 29.50 mg NH3-N /mg chl-a per day and k= 22.12 mg PO43−/mg chl-a per day and saturation constant, Km= 227.68 mg/L of NH3-N and Km= 279.00 mg/L of PO43− with YN= 0.017 mg chl-a/mg NH3-N and Yp= 0.054 mg chl-a/mg PO43−. Thus, these expressions suggested that PO43− produced three time more chl-a than NH3-N produced chl-a during phycoremediation process by Scenedesmus sp. The outcomes from this study suggested that the Scenedesmus sp. bioreactor system is effective with >96% for the organic contents and nutrients removal from WMWW. This study may reduce environmental problem especially for aquatic and beneficial to authorities to produce national guiding principle of WMWW treatment.

Item Type: Thesis (Doctoral)
Subjects: T Technology > TP Chemical technology > TP200-248 Chemicals: Manufacture, use, etc.
Divisions: Faculty of Civil Engineering and Built Environment > Department of Civil Engineering : Water and Environmental Engineering
Depositing User: Mrs. Sabarina Che Mat
Date Deposited: 22 Jun 2021 07:58
Last Modified: 22 Jun 2021 07:58
URI: http://eprints.uthm.edu.my/id/eprint/103

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