Isolation and optimization of phenantherene degradative bacteria from municipal sludge for PAHs bioremediation

Othman, Norzila and Hussain, Noor Hana and Abdul Talib, Suhaimi (2010) Isolation and optimization of phenantherene degradative bacteria from municipal sludge for PAHs bioremediation. In: 3rd Southeast Asian Natural Resources And Environmental Management , 3-5 August 2010, Promende Hotel, Sabah.

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Abstract

Polvcvclic aromatic hydrocarbons (PAHs) are ubiquitous compounds in the environment that originate from natural and anthropogenic pyrolysis of organic matter. It also can cause great ™ n m e n t a l concern because of their persistent, toxicity, mutagenicity and carcinogenicity The USEPA has identified 16 PAHs compounds as priority pollutants whose level in industrial effluents require monitoring. Possible fates for PAHs released into the environment include volatilization, p h ^ - oxidation, chemical oxidation, bioaccumulation and adsorption on soil particles. The principle process for the successful removal and elimination of PAHs from contaminated environment is micr°bud degradation. Many studies from temperate countries had reported on biodegradation of PAHs bin limited information could be found on tropical region. This study is earned out to isolate P * " ^ ™ degradative bacteria from municipal sludge and to optimize their degradation condition for bioremediation purposes. Selective enrichment method using shaken liquid media, namely, minimal medium was a d ^ d to isolate bacteria capable of degrading ph^anthr^e. Thc proc^s mvo ves providing conditions suitable for the growth of bacteria capable of metabohzmg the P h ^ t o ^ Bacterial strains were enriched from municipal sludge sample in minimal media broth supplemented with 0 1 % phenanthrene as the sole carbon source. The mixed cultures were grown, at 30 Con a rotary shaker at 150 rpm for two months. Four parameters including the concentrationi of bacteria, concentration of phenanthrene, pH and temperature were selected to determine their effects^ on the degradation of phenanthrene by the isolated bacteria strains. Several bactenal stmms were isolated through enrichment and one strain, Corynebacterium uroalyticum that was tentatively identifi^ by Ae Biolog system, demonstrated a high removal rate of phenanthrene over oth* Strang Following one cteylag phase more than 85% of phenanthrene degraded after 2 weeks incubation. M a ^ u m rate of phenanthrene removal occurred in the culture containing 100 mgL"1 of phenanthrene. Media at a pH 7 0 was more favourable for the degradation of phenanthrene by Corynebacterium uroalyticum. CfctiS Temperature was determined as 30°C. The isolated Corynetactenurn demonstrated to be a feasible strain for degradation of phenanthrene at a neutral pH, 30°C even up to a phenanthrene concentration of 100 mgL".

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:biodegradation; bioremediation; isolation; optimization; phenanthrene
Subjects:T Technology > TD Environmental technology. Sanitary engineering > TD785-812 Municipal refuse. Solid wastes
Divisions:Faculty of Civil and Environmental Engineering > Department of Water and Environment Engineering
ID Code:1848
Deposited By:Faizul Sahari
Deposited On:05 Oct 2011 10:20
Last Modified:05 Oct 2011 10:20

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