Isolation and optimization of napthalene degradative bacteria

Othman, Norzila and Hussain, Noor Hana and Abd Karim, Ahmad Tarmizi and Abdul Talib, Suhaimi (2009) Isolation and optimization of napthalene degradative bacteria. In: International Conference on Sustainable Infrastructure and Built Environment in Developing Countries, 2-3 November 2009, West Jawa, Indonesia.

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Abstract

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment and cause great environmental concern because of their persistent, toxicity, mutagenicity and carcinogenicity. Therefore they are listed by the United States Environmental Protection Agency (USEPA) as priority pollutants. The principle process for the successful removal and elimination of PAHs from contaminated environment is microbial degradation. Many studies from temperate countries had reported on biodegradation of PAHs but limited information could be found on tropical region. This study is carried out to isolate PAH degradative bacteria from municipal sludge and to optimize their degradation condition including pH, temperature and napthalene concentration required for bioremediation purposes. Several bacterial strains were isolated through enrichment and one strain, Micrococcus diversus that was tentatively identified by the Biolog system, demonstrated a high removal rate of naphthalene over other strains. Following one day lag phase more than 85% of naphthalene degraded after six days incubation. Maximum rate of naphthalene removal occurred in the culture containing 100 mgU' of naphthalene. Media at a pH 7.0 was more favourable for the degradation of napthalene by Micrococcus diversus. Optimal temperature was determined as 30°C. The isolated Micrococcus diversus demonstrated to be a feasible strain for degradation of napthalene at a neutral pH, 30°C even up to a napthalene concentration of 100 mgL'1.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:biodegradation; bioremediation; isolation; napthalene; pptimization
Subjects:T Technology > TD Environmental technology. Sanitary engineering > TD1067-9999 Hazardous substances and their disposal
Divisions:Faculty of Civil and Environmental Engineering > Department of Water and Environment Engineering
ID Code:2019
Deposited By:Khairunnisa Ahmad
Deposited On:05 Oct 2011 12:03
Last Modified:05 Oct 2011 12:03

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