Degradation of polycyclic aromatic hydrocarbon by pure strain isolated from municipal sludge: synergistic and cometabolism phenomena

Othman , N. and Hussain, N. and Abdul-Talib, S. (2011) Degradation of polycyclic aromatic hydrocarbon by pure strain isolated from municipal sludge: synergistic and cometabolism phenomena. In: International Conference on Environment (ICENV2010), 13-15 Disember 2010, Pulau Pinang.

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Abstract

Polycyclic aromatic hydrocarbons are classed as potentially hazardous chemicals of environmental and health concern. Some PAHs exhibit carcinogenic and mutagenic properties and are listed by the USEPA as priority pollutants. The principle process for the successful removal and elimination of PAHs from contaminated environment is microbial degradation. Many studies from temperate countries had reported on biodegradation of PAHs using microbial from petroleum contaminated area but limited information could be found from other medium. Thus, the previous studies have not investigated in detail the interaction among PAHs during biodegradation. This study was carried out to isolate PAHs degrading bacteria from municipal sludge and to determine the effect of enrichment substrate and PAH degradative bacteria activities during degradation of PAHs. Several microorganisms were isolated from municipal sludge for their ability to mineralize PAHs as sole carbon and energy source. Enrichment method process utilised single PAHs as a sole source of carbon. The pure culture was identified using the Biolog system. Biodegradation experiment was performed using 250 ml reactor flasks containing 50ml minimal media, 10% of bacterial inoculum and the specific PAHs. All samples were tested at an initial pH of 7.0 and temperature controlled at 30oC. The PAHs in the samples were extracted using solid phase microextraction. Extracts were analyzed by Perkin Elmer Clarus 500 gas chromatography. The result of this study showed that isolation and identification process had sucessfully determined a few potential bacteria that able to degrade PAHs. Degradation trend observed in degradation study could be attributed to the different substrate provided during isolation process. Interaction through cometabolism and synergistic occurred for bacteria srains isolated from single substrate.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:biodegradation; degradative bacteria; polycyclic aromatic hydrocarbon
Subjects:T Technology > TD Environmental technology. Sanitary engineering > TD1020-1066 Hazardous substances and their disposal
Divisions:Faculty of Civil and Environmental Engineering > Department of Civil Engineering
ID Code:2043
Deposited By:Normajihan Abd. Rahman
Deposited On:05 Oct 2011 15:30
Last Modified:05 Oct 2011 15:30

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