Othman, Norzila (2010) Polycyclic aromatic hydrocarbons biodegradation using isolated strains under indigenous condition. Doctoral thesis, Universiti Teknologi Malaysia.
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Abstract
The treatment and disposal of domestic sIudge is an expensive and environmentally sensitive problem. It is also a growing problem since sludge production will continue to increase as new wastewzter treatment plants are built due to population increase. The large volume of domestic sIudge produced had made it difficult for many countries including Malaysia to assure complete treatment of the sludge before discharging to the receiving environment. Domestic sludge contains diverse range of pollutants such as pathogen, inorganic and organic compounds. These pollutants are toxic, mutagenic or carcinogenic and may threaten human health. Iiilproper disposal and handling of sludge may pose serious impact to the environment especially on soil and water cycles. Previous studies on Malaysian domestic sludge only reported on bulk parameters and heavy metals. Thus, no study reported on organic micro pollutants, namely, polycylic aromatic hydrocarbons (PAHs). Their recalcitrance and persistence make them problematic environmental contaminants. Microbial degradation is considered to be the primary mechanism of PAHs removal from the environment. Much has been reported on biodegradation of PAHs in several countries but there is a lack of information quantitative on this subject in Malaysia. This study is carried out to understand the nature of domestic sludge and to provide a better understanding on the biodegradation processes of PAHs. The methodology of this study comprised field activities, laboratory work and mathematical modelling. Field activities involved sampling of domestic sludge from Kolej Mawar, Universiti Teknologi MARA, Shah Alam, Selangor. Laboratory activities include seven phases of experimental works. First phase is characterization study of domestic sludge based on bulk parameters, heavy metals and PAHs. Second phase is enrichment and purification of bacteria isolated from domestic sludge using single PAHs and mixed PAHs as growth substrate. This was followed by identification of bacteria using BIOLOG system. The fourth phase focussed on turbidity test to monitor growth rate of the isolated bacteria. Preliminary degradation study involves optimization of the process at different substrate concentration, bacteria concentration, pH and temperature. The optimum conditions established from optimization study were used in degradation study. In biodegradation study, two experimental conditions were performed. These conditions include using bacteria isolated from single PAHs as substrate and bacteria isolated from mixed PAHs. Protein and pH tests were done during degradation study. Final activity is mathematical modelling of the biodegradation process. In general results on bulk parameters are comparable to previous studies. Zinc was the main compound with a mean concentration of 11 96.4 mglkg. PAHs were also detected in all of the samples, with total concentration between 0.72 to 5.36 mglkg dry weight for six PAHs. In the examined samples, phenanthrene was the main compound with a mean concentration of 1.0567 mglkg. The results fiom purification studies of bacteria strains sucessfull isolated 13 bacteria strains fiom single PAH substrate while three bacteria were isolated from the mixed PAHs substrate. Based on bacteria growth rates, only six strains grown on single PAHs and three strains grown on mixed PAHs were used for further studies. Results from the optimization study of biodegradation indicated that maximum rate of PAHs removal occurred at 100 mg~-' of PAHs, 10% bacteria concentration, pH 7.0 and 30°C. The results showed that bacteria grown on lower ring of PAHs are not able to grow on higher ring of PAHs. As for example Micrococcus diversus grown on napthalene as sole carbon source was unable to degrade other PAHs like acenapthylene, acenapthene, fluorene, phenanthrene and antlracene. In the case of bacteria isolated from mixed PAHs, the results showed that most of the napthalene was degraded by isolated strains with the highest average degradation rate followed by acenapthylene, acenapthene, fluorene, phenanthrene and anthracene. 377$ 3(53867$.$$1�781.8�781�$0,1$+ D4ff + c\,cpda~ition trends were observed in the study could be attributed to the different subsr , i,lo\~ir 'Led during isolation process. Interaction through cometabolism and synergistic ocolq bacteria strains isolated from single substrate. Thus, only synergistic interaction was oL, :a 77ed for bacteria isolated from mixed substrate. Corynebacterium urolyticum re\e;;ed I,, be the best strain in degrading PAHs. The experimental results have led to a model conccl~t desclibing I'AHs degradation.
| Item Type: | Thesis (Doctoral) |
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| Subjects: | Q Science > QP Physiology Q Science > QP Physiology > QP501-801 Animal biochemistry |
| Depositing User: | Mrs. Sabarina Che Mat |
| Date Deposited: | 03 Feb 2022 01:47 |
| Last Modified: | 03 Feb 2022 01:47 |
| URI: | http://eprints.uthm.edu.my/id/eprint/2209 |
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