The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain

Kamilah, Hanisah and Al-Gheethi, Adel and Yang, Tajul Aris and Sudesh, Kumar (2018) The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain. Arabian Journal for Science and Engineering, 43. pp. 3453-3463. ISSN 2193-567X

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Official URL: https://doi.org/10.1007/s13369-018-3118-1

Abstract

Waste cooking oil (WCO) is of increasing interest as an inexpensive feedstock to produce biodegradable plastic, poly(3- hydroxybutyrate) [P(3HB)]. In the present study, palm oil-basedWCO(PO-WCO) was obtained from nine different locations. Palm oil-based fresh cooking oil (PO-FCO) and PO-WCO were characterised via proximate and physicochemical analysis, prior to being used as carbon sources for the biosynthesis of P(3HB) using Cupriavidus necator H16. It was shown that the free fatty acid, peroxide value, and saturated compounds in all batches of PO-WCO were higher compared to those in the PO-FCO. The cells produced 60–80 wt% P(3HB) with dry cell weight of 14–17 g/L. The weight average molecular weight (Mw) was found to be 1.8 × 106 Da with a polydispersity (Mw/Mn) of 2.7 when PO-WCO was used as the carbon source. The PO-WCO was found to be suitable to be used as a sustainable carbon source for cell growth and P(3HB) biosynthesis.

Item Type:	Article
Uncontrolled Keywords:	Biomaterial; Waste; Cooking oil; P(3HB); C. necator H16
Subjects:	T Technology > TD Environmental technology. Sanitary engineering > TD201-500 Water supply for domestic and industrial purposes > TD429.5-480.7 Water purification. Water treatment and conditioning. Saline water conversion
Divisions:	Faculty of Civil Engineering and Built Environment > Department of Civil Engineering : Water and Environmental Engineering
Depositing User:	Miss Afiqah Faiqah Mohd Hafiz
Date Deposited:	16 Nov 2021 06:57
Last Modified:	16 Nov 2021 06:57
URI:	http://eprints.uthm.edu.my/id/eprint/3125

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