Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang)

Rahim, A. Che and Abu Bakar, Mohd Fadzelly and Kassim, Kartinee and Stanslas, Johnson and Wan Mohamad Zain, Wan Nor I'zzah Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang). Asian Journal of Chemistry, 30 (10). pp. 2273-2276. ISSN 0975-427X

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Bambangan (Mangifera pajang) has been shown to exhibit anticancer activity. One of the major bioactive compounds present in the methanol extract of kernel of M. pajang is methyl 3,4,5-trihydroxybenzoate (methyl gallate). The present study was conducted to evaluate the cytotoxic activity of this compound against selected cancer cell lines such as hormone dependent breast cancer (MCF-7), nonhormone dependent breast cancer (MDA-MB-231), prostate cancer (PC-3), pancreatic cancer cell (CP-2) and colon cancer (HCT-116) cell lines. Methyl gallate was isolated from methanol extract by using column chromatography and the compound was further confirmed by using NMR, GC-MS analysis and comparison of spectral data of the isolated data with published report. The cytotoxicity of the compound was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay at concentrationsranging from 0 to 100 µM. The results showed that the compound only induced cytotoxicity in MCF-7 and PC-3 cell lines with IC50 values of 54.7 ± 4.73 and 97.6 ± 4.04 µM, respectively. Furthermore, Capan 2, MDA-MB-231 and HCT-116 showed no inhibition towards the cell proliferation after the treatment with compound (IC50 values more than100 µM). Thus, the compound isolated from kernel of M. pajang exhibited selective cytotoxic activity against selected cancer cell lines.

Item Type: Article
Subjects: T Technology > T Technology (General)
Divisions: Faculty of Applied Science and Technology > Department of Technology and Natural Resources
Depositing User: UiTM Student Praktikal
Date Deposited: 09 Jan 2022 07:29
Last Modified: 09 Jan 2022 07:29

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