Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island

Muhammad Lutfi, Fatin Najihah and Mohamed Rehan, Maryam and Kamarudin, Kamarul Rahim and Che Zahri, Mohd Zulhafiz and Mohd Noor, Hanina and Mat Radzi, Salina (2018) Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island. Malaysian Applied Biology, 47 (2). pp. 95-101. ISSN 0126-8643

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Abstract

Holothuria (Mertensiothuria) leucospilota is the most abundant sea cucumber species in Malaysia. This study aimed to identify bacteria isolated from the external and internal body parts of H. leucospilota collected from the coastal water of Pangkor Island, Perak, Malaysia. A total of 26 bacterial samples were isolated using streak plate method from eight body parts of two fresh H. leucospilota specimens and from the surrounding surface sediments and seawater. Identification of the bacterial isolates was based on microscopic examination, 16S rDNA amplification and phylogenetic analysis using the neighbour-joining method. Three genera of bacteria were identified namely Vibrio, Bacillus, and Acinetobacter. The genus Vibrio was found to be the main bacterial group associated with the H. leucospilota specimens from Pangkor Island.

Item Type:	Article
Uncontrolled Keywords:	Holothuria leucospilota; Bacteria; 16S rDNA; Pangkor Island
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Applied Science and Technology > Department of Technology and Natural Resources
Depositing User:	UiTM Student Praktikal
Date Deposited:	23 Jan 2022 08:17
Last Modified:	23 Jan 2022 08:17
URI:	http://eprints.uthm.edu.my/id/eprint/5802

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