Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose

Z Abidin, Munira and Kourmentza, Constantina and K Karatzas, Andreas and Niranjan, Keshavan (2019) Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose. Journal of Chemical Technology and Biotechnology, 94 (8). pp. 2529-2536. ISSN 0268-2575

[img] Text
AJ 2020 (207).pdf
Restricted to Registered users only

Download (576kB) | Request a copy


Background: n,n′-diacetylchitobiose (glc n ac2) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre-treatment process using steam under pressure in an autoclave was employed to facilitate subsequent enzymatic hydrolysis of chitin with chitinase from streptomyces griseus. Results: pre-treatment of chitin with 0.05mol l−1 sodium acetate buffer (p h= 6.0) at 121 ∘c for 60 min, followed by enzymatic hydrolysis involving24 hincubation,were foundtobethebest conditions for producing glc n ac2. the glc n ac2 thus obtainedwas tested regarding its antimicrobial activity against gram-negative and gram-positive strains and showed minimum inhibitory concentrations at 5 and 10% (w/v) against escherichia coli k-12 and listeria monocytogenes 10403 s, respectively. Conclusions: the extent of swelling and crystallite size of chitin increased with pre-treatment residence time, and enhanced the rate of subsequent hydrolysis using chitinase. © 2019 society of chemical industry

Item Type: Article
Uncontrolled Keywords: thermal pre-treatment; enzymatic hydrolysis; GlcNAc2; antimicrobial activity
Subjects: T Technology > TP Chemical technology
Divisions: Faculty of Applied Science and Technology > Department of Technology and Natural Resources
Depositing User: Miss Afiqah Faiqah Mohd Hafiz
Date Deposited: 26 Jan 2022 08:53
Last Modified: 26 Jan 2022 08:53
URI: http://eprints.uthm.edu.my/id/eprint/6136

Actions (login required)

View Item View Item