Design and development of coliform bacteria detection system integrated with microfluidic and optical absorbance measurement device

Md Salih, Nurulazirah (2016) Design and development of coliform bacteria detection system integrated with microfluidic and optical absorbance measurement device. Masters thesis, Universiti Tun Hussein Onn Malaysia.

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Abstract

The detection of coliform bacteria which contain the disease-causing microorganism is a useful indication for water contamination. This primary indication is important for diagnosis of infectious disease, as well as for countermeasure to potential biological threats. Currently, the emerging of technology in molecular biology research and industry is in demand for portable and miniaturized system. This project involved with design and development of microfluidic and optical absorbance measurement device for coliform bacteria detection system. Suitable microfluidic design was simulated in the COMSOL Multiphysics software. The microfluidic device was designed for coliform bacteria sample using optical detection approach. The microfluidic device was fabricated with glass and polydimethylsiloxane (PDMS) material using photolithography, replica moulding (soft lithography), and oxygen plasma bonding techniques. Then, the optical absorbance measurement device for coliform bacteria detection was developed based on the optical absorbance theory. The device was constructed using 470 nm blue light emitting diode (LED), photo detector, ARDUINO microcontroller, liquid crystal display (LCD), and mechanical elements. This project had successfully developed a prototype which integrates the PDMS-glass based microfluidic and optical absorbance measurement device. The absorbance measurement from the prototype and colony number of the coliform bacteria samples were collected and analyzed. The collected data was used for the prototype programme. The final analysis had indicated that the developed prototype was able to detect the coliform bacteria in suspension at the lowest detection of 13,400 CFU/ml.

Item Type:Thesis (Masters)
Subjects:T Technology > TJ Mechanical engineering and machinery > TJ836-935 Hydraulic machinery
ID Code:8800
Deposited By:Mr. Mohammad Shaifulrip Ithnin
Deposited On:06 Feb 2017 15:06
Last Modified:06 Feb 2017 15:06

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