Transforming growth factor- β1 and β3 manipulation of HaCaT keratinocyte attachment to a 12.5μm fibronectin patterned surface illustrated by widefield surface plasmon resonance microscopy

Charles, P. M. and Denyer, M. C. T. and Abdul Jamil, M. M. (2008) Transforming growth factor- β1 and β3 manipulation of HaCaT keratinocyte attachment to a 12.5μm fibronectin patterned surface illustrated by widefield surface plasmon resonance microscopy. In: 4th Kuala Lumpur International Conference on Biomedical Engineering 2008 (BIOMED 2008), 25–28 June 2008, Kuala Lumpur.

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Official URL: https://link.springer.com/chapter/10.1007/978-3-54...

Abstract

The influence of Transforming Growth Factor- β1 (TGF) and β3 on keratinocyte surface attachment has yet to be fully elucidated. Widefield Surface Plasmon Resonance (WSPR) microscopy allows changes in the arrangement of focal contacts to be visualised. HaCaT keratinocytes were plated on a 12.5μm fibronectin micropattern with TGF-β1 or β3 and WSPR photomicrographs were taken. The effects of TGF-β3 inhibition was attenuated by the 12.5μm fibronectin micropattern. TGF-β1 promoted an arrangement of focal contacts consistent with both stationary and migratory HaCaT keratinocyte phenotypes. The arrangement and location of the focal contacts supports literature suggesting TGF-β3 promotes a migratory phenotype whilst TGF-β1 in excess impairs reepithelialisation.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:transforming growth factor; TGF-β1; TGF-β3; WSPR; high resolution; keratinocytes; focal contacts
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Faculty of Electrical and Electronic Engineering > Department of Electronic Engineering
ID Code:9631
Deposited By:Mr. Mohammad Shaifulrip Ithnin
Deposited On:24 Dec 2017 20:50
Last Modified:24 Dec 2017 20:50

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