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Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis

Wan Azelee, Nur Izyan and Md Jahim, Jamaliah and Ismail, Ahmad Fauzi and Mohamad Fuzi, Siti Fatimah Zaharah and A. Rahman, Roshanida and Ghazali, Nazlee Faisal and Md Illias, Rosli (2016) Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis. American Journal of Agricultural and Biological Sciences, 11 (2). pp. 54-66. ISSN 15574997

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Abstract

An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production for food additives and nutraceuticals industries. In this study, xylanase hydrolysis of pretreated hemicellulosic kenaf (Hibiscus cannabinus) for substantial hemicellulose hydrolysis was demonstrated. Experiments were performed primarily to investigate the effect of several critical reaction conditions towards the enzymatic hydrolysis of the pre-treated kenaf. The effects of various ranges of reaction conditions (substrate loading, xylanase loading, pH, temperature and time) were systematically and thoroughly studied. The study of hemicellulosic kenaf conversion using single enzyme was proven to effectively yield up to 59% of conversion after 48 h incubation using 3% (w/v) of substrate loading and 400 U of xylanase loading at 50°C in pH 4.0 buffer systems. The use of a single xylanase on the pre-treated kenaf was a key parameter in understanding the action of the main hemicellulose degrading enzyme towards hydrolysing the hemicellulose backbone of the pre-treated kenaf. Enzyme feeding strategy was also conducted and the results revealed that a batch feeding strategy of 400 U xylanase loading yielded the highest hemicellulose hydrolysis compared to the other fed batch enzyme feeding strategy. It was concluded that each reaction conditions and enzyme feeding strategy gave a significant impact towards the enzymatic hydrolysis and the use of optimum reaction conditions resulted in a high product yield at the end of the reaction. With the discovery of the importance of each reaction conditions involved in a hydrolysis, this study highlights the need for a comprehensive investigation on the reaction conditions using a mixture of enzymes towards achieving a complete hydrolysis with a higher product yield.

Item Type: Article
Uncontrolled Keywords: Enzymatic hydrolysis; hemicellulose; kenaf; reaction conditions; xylanase
Subjects: T Technology > TP Chemical technology > TP1-154 Chemical technology
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 03 Jul 2018 11:12
Last Modified: 03 Jul 2018 11:12
URI: http://eprints.uthm.edu.my/id/eprint/10124
Statistic Details: View Download Statistic

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