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High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis

Wan Azelee, Nur Izyan and Md Jahim, Jamaliah and Ismail, Ahmad Fauzi and Mohamad Fuzi, Siti Fatimah Zaharah and Md Illias, Rosli (2016) High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis. Industrial Crops and Products, 81. pp. 11-19. ISSN 09266690

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Advancement in industrial biotechnology offers potential opportunities for economic utilization of agro-industrial biomass such as kenaf (Hibiscus cannabinus) for the production of value-added products. Thiswork was conducted to evaluate an enzymatic hydrolysis of kenaf stem (that has been pretreated)for xylooligosaccharides (XOS) production. Pretreated kenaf stems were subjected to two enzymatichydrolysis approaches which are single xylanase (Xyn2) and a mixture of xylanase:arabinofuranosidase(Xyn2:AnabfA). Central Composite Design was used to optimize the enzymatic hydrolysis conditions onthe pretreated kenaf stem to achieve maximum hemicellulose conversion. Under the optimized hydroly-sis conditions, hemicellulose conversion (95.03%) with the highest XOS yield of 351.46 mg/g was achievedat 40◦C (pH 4.0) after 48 h incubation with Xyn2:AnabfA ratio of 400 U:10 U. Xylobiose (135.42 mg/g) isthe main XOS product with xylotriose (102.04 mg/g) being the second highest. The high production ofXOS yield from the pretreated kenaf stem demonstrated the biotechnological potential of the kenaf inthe future.

Item Type: Article
Uncontrolled Keywords: Arabinofuranosidase; enzyme mixture; hemicellulose; xylanase; xylooligosaccharides
Subjects: T Technology > TP Chemical technology > TP1-154 Chemical technology
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 03 Jul 2018 11:11
Last Modified: 03 Jul 2018 11:11
URI: http://eprints.uthm.edu.my/id/eprint/10128
Statistic Details: View Download Statistic

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