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Effect of temperature condition on xylanase production by immobilizing E. coli onto graphene oxide

Nor Ashikin, Nur Atiqah Lyana and Che Man, Rohaida and Low, Kheng Onn and Md Illias, Rosli and Md Fuzi, Siti Fatimah Zaharah (2016) Effect of temperature condition on xylanase production by immobilizing E. coli onto graphene oxide. In: International Conference on Biosciences & Medical Engineering (ICBME2016), 10th –11th November 2016, Universiti Teknologi Malaysia.

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Abstract

The targeting of recombinant proteins for excretion into culture medium presents significant advantages over cytoplasmic expression. However, during the excretion of recombinant protein, caution must be taken in order to avoid cell lysis due to pressure build-up through overproduction of the expressed recombinant protein in the periplasmic space. The recombinant E. coli cells were immobilized on the graphene oxide with temperature 30 degree celcius. The results presented showed that the immobilized cell is a promising method for high enzyme excretion and plasmid stability with less occurrences of cell lysis. The immobilized cell exhibited a 7% increase in the xylanase excretion 39% reduction of cell lysis compared with free cells.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Immobilization; graphene oxide; xylanase
Subjects: Q Science > QR Microbiology
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 13 Aug 2018 03:29
Last Modified: 13 Aug 2018 03:29
URI: http://eprints.uthm.edu.my/id/eprint/10163
Statistic Details: View Download Statistic

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