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Optimization of low amplitude pulse electric field exposure on colon cancer cell for wound healing application

Buhari, Mamman Hassan (2017) Optimization of low amplitude pulse electric field exposure on colon cancer cell for wound healing application. PhD thesis, Universiti Tun Hussein Onn Malaysia.

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Abstract

Pulse electric field (PEF) is a method of creating transient pores in the cell membrane by exposing it to a high voltage electric field of short duration. Beside having the effect of opening pores in the membrane, PEF can change the cytoskeletal restructuring of the cell, which has an impact on the cell morphological properties and signalling pathways. There was evidence that extracellular matrix (ECM) protein and transforming growth factor beta (TGF-β) can influence cellular behaviours. Similarly, PEF was also found to modulate the cellular behaviours. However, a combined influence of PEF and ECM on cellular behaviour which could further enhances the cellular processes for wound healing and tissue engineering application has not yet been investigated. Therefore, the aim of this study is to examine the effect of PEF in combination with ECM or TGF-βs on cellular behaviour such as attachment proliferation, adhesion, migration and alignment of HT29 cell line monolayer in vitro. Cell-surface adhesion was studied via trypsinization assay in which the time taken for cells to detach from the monolayer would give the means of assessing the strength of adhesion. Cell migration was studied by creating a wound model on a confluence monolayer of the cell in vitro. Cell alignment was investigated via micro contact printing techniques. The PEF was found to increase the attachment, proliferation and migration rate of the HT29 cell by 67.6, 27.1 and 108% respectively and decrease the adhesion strength of the cells by 29.2%. The results also show that 600V/cm and 5ms electric parameter could potentially reduce the unpleasant sensation associated with the standard electric field parameter currently in used in electrochemotherapy. The results of the cell alignment to the ECM proteins pattern have shown that PEF has improved the HT29 cell alignment by more than 40%. Similarly, the PEF was found to increase the proliferation and decrease the adhesion rate of cells in a TGF-β3 supplement by 50.2 and 24.1% respectively. Thus, the outcomes of this research revealed that the use of PEF in the presence of ECM protein have potential implication in wound healing application (by increasing the rate of cell migration and decreasing cell adhesion).

Item Type: Thesis (PhD)
Subjects: T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK2896-2986 Production of electricity by direct energy conversion
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 24 Jun 2018 01:39
Last Modified: 24 Jun 2018 01:39
URI: http://eprints.uthm.edu.my/id/eprint/10178
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