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Lab-on-chip microfluidic system for single cell mass measurement

Rahman, Md. Habibur and Ahmad, Ida Laila and SayahKarajy, Mostafa and Ahmad, Mohd Ridzuan and Masahiro, Nakajima and Toshio, Fukuda (2014) Lab-on-chip microfluidic system for single cell mass measurement. In: 2014 IEEE Conference on Biomedical Engineering and Sciences, 8–10 Dec 2014, Miri, Sarawak.

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In this paper, we presented a lab-on-chip microfluidic system for single cell mass measurement. Single cell mass has been related with drag force exerted on cell and Newton law of motion. Drag force has been generated using pressure driven syringe micropump. Motions of the cells were measured using optical observation under an inverted microscope. We have calibrated the approach using known mass of polystyrene microbeads with a diameter of 5.2 μm. Our experimental results showed that, mass of single microbead is 88.9 fg, which is very close to the theoretical mass (77.3 fg) of the particle. From which it is believed, our approach is suitable for single particle’s mass measurement. We used Saccharomyces cerevisiae baker’s yeast cell as a cell sample. Yeast cells have been cultured in our laboratory using YPD medium. For the diameter of 4 μm cell, mass of single cell was measured as 1.9 pg which is very consistent with previously reported single yeast cell mass (1-3 pg).

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Microfluidic device; drag force; cell culture; single yeast cell mass
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Electrical and Electronic Engineering > Department of Electronic Engineering
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 25 Sep 2018 08:20
Last Modified: 25 Sep 2018 08:20
URI: http://eprints.uthm.edu.my/id/eprint/10383
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