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Study the effectiveness of HRP/PANI eletrode as sensor to detect level of H2O2 in urine

O., Siti Amira and R., Shahidan (2016) Study the effectiveness of HRP/PANI eletrode as sensor to detect level of H2O2 in urine. Malaysian Journal of Public Health Medicine, 16 (4). p. 79. ISSN 16750306

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The level of hydrogen peroxide (H2O2) in urine has been suggested as a potential biomarker of whole body oxidative stress, but issues of stability, reproducibility and biological variation still be issues. Immobilise enzymes can offer many advantages over their soluble forms making this is a topic of active research in enzyme technology for industrial applications. Horseradish Peroxidase (HRP) was used extensively in molecular biology applications primarily for its ability to amplify a weak signal and increase the detectability of a target molecules. Recently class of conducting polymers are used as a polymeric support. Among the family a conducting polymer, polyaniline (PANI) has intensively been used for various reason because of its unique properties such as ease of synthesis by chemical or electrochemical oxidative polymerization of the monomers, role as a polymeric support and also have potential in various field. This study intends to see the effectiveness of HRP when it is deposited onto PANI layer using electrodeposition method to detect level of H2O2 in urine by potentiostatic technique. The characterization was done using UV-Visible and FTIR. For U.V.-visible, HRP/PANI shows a two sharp absorbtion peaks at ~310 nm and ~810 nm. This is supported by FTIR result that proves the existence of shifted peak because of interaction between HRP and PANI. Detection response shown by HRP/PANI electrode towards H2O2 gave a response time as early as 0.01 second. This gave promising in applications for detection of concentration hydrogen peroxide level in urine that useful for clinical applications.

Item Type: Article
Uncontrolled Keywords: HRP; PANI; H2O2; urine
Subjects: R Medicine > R Medicine (General)
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 13 May 2019 01:12
Last Modified: 13 May 2019 01:12
URI: http://eprints.uthm.edu.my/id/eprint/11186
Statistic Details: View Download Statistic

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