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New medium for isolation of bacteria from cement kiln dust with a potential to apply in bio-concrete

A. F., Alshalif and J., M. Irwan and N., Othman and Al-Gheethi , A. (2017) New medium for isolation of bacteria from cement kiln dust with a potential to apply in bio-concrete. In: 4th International Conference on Civil and Environmental Engineering for Sustainability (IConCEES 2017), 4–5 December 2017, Langkawi, Malaysia.

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Abstract

The present study aimed to introduce a new isolation medium named kiln dust medium (KDM) for recovering of bacteria from cement kiln dust with high pH (>pH 11) without the need for nutrients additives. The cement kiln dust samples were collected from five different areas of Cement Industries of Malaysia Berhad (CIMA). The bacterial isolates were recovered on KDM by direct plating technique. The chemical components for all collected samples were identified using X-ray fluorescence (XRF). The primary identification for the bacterial isolates indicated that these bacteria belongs to Bacillus spp. Based on the morphological characteristics. The growth curve of the bacterial strains was monitored using the optical density (OD) with 650 nm wavelength, which in role confirmed that all isolated bacteria had the ability to grow successfully in the proposed medium. The ability of the bacterial strains to grow at high pH reflects their potential in the bio-concrete applications (aerated and non-aerated concrete). These findings indicated that the cement kiln dust samples from Cement Industries represent the most appropriate source for bacteria used in the bioconcrete.

Item Type: Conference or Workshop Item (Paper)
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA401-492 Materials of engineering and construction. Mechanics of materials
Divisions: Faculty of Civil and Environmental Engineering > Department of Structural and Material Engineering
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 29 Aug 2019 03:41
Last Modified: 29 Aug 2019 03:41
URI: http://eprints.uthm.edu.my/id/eprint/11460
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