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Optimizing of pharmaceutical active compounds biodegradability in secondary effluents by β-lactamase from Bacillus subtilis using central composite design

Al-Gheethi, Adel Ali Saeed and Noman, Efaq Ali and Radin Mohamed, Radin Maya Saphira and Ismail, Norli and Abdullah, Abd Halid and Mohd Kassim, Amir Hashim (2018) Optimizing of pharmaceutical active compounds biodegradability in secondary effluents by β-lactamase from Bacillus subtilis using central composite design. Journal of Hazardous Materials. pp. 883-894.

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Abstract

Biodegradation of pharmaceuticals active compounds (PACs) in secondary effluents by using B. subtilis 2012WTNC as a function of β-lactamase was optimized using response surface methodology (RSM) designed by central composite design (CCD). Four factors including initial concentration of bacteria (1–6 log10 CFU mL−1), incubation period (1–14 days), incubation temperature (20–40 °C) and initial concentration of PACs (1–5mg L−1) were investigated. The optimal operating factors for biodegradation process determined using response surface methodology (RSM) was recorded with 5.57 log10 CFU mL−1 of B. subtilis, for 10.38 days, at 36.62 °C and with 4.14 mg L−1 of (cephalexin/amoxicillin) with R2 coefficient of 0.99. The biodegradation was 83.81 and 93.94% respectively. The relationship among the independent variables was significant (p < 0.05) with 95% of confidence level at the best operating parameters. The bioassay for PACs after the degradation process revealed that no residual antibiotic activity was detected of amoxicillin and cephalexin against E. coli and S. aureus after degradation using B. subtilis which reflects the higher potential of bacteria to biodegrade PACs in secondary effluents. B. subtilis has the potential for biodegradation of PACs in the secondary effluents.

Item Type: Article
Uncontrolled Keywords: B. subtilis; amoxicillin; cephalexin; bioactivity; degradation pathway
Subjects: H Social Sciences > HD Industries. Land use. Labor > HD9000-9999 Special industries and trades > HD9665-9675 Pharmaceutical industry
Depositing User: Mr Abdul Rahim Mat Radzuan
Date Deposited: 31 Oct 2019 02:44
Last Modified: 31 Oct 2019 02:44
URI: http://eprints.uthm.edu.my/id/eprint/11916
Statistic Details: View Download Statistic

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