UTHM Institutional Repository

Tapered microfluidic device for multi-particle separation based on sedimentation principle

Ahmad, Ida Laila (2017) Tapered microfluidic device for multi-particle separation based on sedimentation principle. Doctoral thesis, Universiti Tun Hussein Onn Malaysia.

[img]
Preview
Text
24p IdaLailaPE133014d17ttt.pdf

Download (1MB) | Preview

Abstract

This thesis presents a label-free tapered microfluidic device for a passive multi-particle separation. Separation process plays a significant role in various industries for example, biomedical diagnostic, food processing and substance purification. The growing needs for continuous separation process lead to the creation of numerous microfluidic based separation devices. Currently, microfluidic based separation devices are associated with limitations in terms of design complexity, sample purity and separation throughput. Therefore, a simple novel passive tapered microfluidic separation device with various taper angles (6°, 12°, 20° and 25°) is proposed for high purity separation of biological and non-biological samples. The device utilizes coupling mechanism between hydrodynamic separations along with sedimentation effect for enhancement of sample purity. Computer-aided design software was employed during design stage while Finite Element Analysis (FEA) software was used for device design’s optimization. The device was fabricated using a soft lithography technique and was characterized in terms of physical dimensions and leakage conditions. Size based separation simulations using FEA were carried out for 3 μm and 10 μm diameters polystyrene (PS) microbeads samples as well as a mixture of 3 μm PS microbeads and Human Cervical Epithelial Carcinoma (HeLa) cells. Through FEA simulations, larger particles were collected at Outlet 1 and small particles were collected at Outlet 2 using 20° and 25° tapered devices. Furthermore, experimental tests were conducted with similar settings and samples as in the simulations. Successful multi-particle separations were observed using 20° and 25° tapered devices at 0.5 to 3.0 μl/min flow rates. These results were in agreement with simulation results obtained. Highest purity of 98% was achieved for both samples with the use of 3.0 μl/min flow rate. As a conclusion, a passive tapered microfluidic device capable of multi-particle separation at high sample purity was developed.

Item Type: Thesis (Doctoral)
Subjects: T Technology > TJ Mechanical engineering and machinery > TJ836-935 Hydraulic machinery
Divisions: Faculty of Electrical and Electronic Engineering > Department of Electrical Technology
Depositing User: Sabarina Che Mat
Date Deposited: 30 Sep 2019 02:50
Last Modified: 30 Sep 2019 02:51
URI: http://eprints.uthm.edu.my/id/eprint/12046
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year