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Dry phase detection of ultra thin multilayer poly electrolyte films using spectral reflectance technique

Majeed, Uzair and Mustafa, Mohd Kamarulzaki and Nayan, Nafarizal (2013) Dry phase detection of ultra thin multilayer poly electrolyte films using spectral reflectance technique. In: Prosiding Seminar Kebangsaan Aplikasi Sains dan Matematik 2013 (SKASM 2013) , 29-30 Oktober 2013, Batu Pahat, Johor .

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Abstract

Surface modification with polyelectrolyte multilayer films appears as a versatile tool for immunoassay preparation in biosensing application. The technique of electrostatic self assembly (ESA), also known as poly electrolyte self assembly (PESA) or electrostatic layer by layer (LBL) deposition is based upon electrostatic interaction between polymers containing cation and anion groups. ESA method became extremely popular and within the last decade was adopted and hrther developed in many research laboratories. In present work,poly(allylamine hydrochloride) (PAH) and poly(sodium Cstyrenesulfonate) (PSS) were employed to build up multilayers on oxidized silicon substrates. An incubation period about 10 minutes is given to allow the binding take place before it was dried by nitrogen flow. The shift in reflectancefi-om deposited polyelectrolyte layers is measured by spectral reflectance (SR) technique after each deposition. It was verified that this technique isable to measure down to angstrom level and suitable for label fiee optical detection for thin film in dry phase.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: electrostatic self-assembly; spectral reflectance; layer by layer deposition; dry phase detection; optical detection
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Applied Science and Technology > Department of Science and Mathematics
Depositing User: Normajihan Abd. Rahman
Date Deposited: 21 Nov 2013 07:50
Last Modified: 21 Jan 2015 07:59
URI: http://eprints.uthm.edu.my/id/eprint/4526
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