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Enumeration of salmonella in compost material by a nonculture based method

Mohamed Sunar, Norshuhaila and Stewart, Douglas and Edward, Stentiford and fletcher, Loiuse (2009) Enumeration of salmonella in compost material by a nonculture based method. In: Proceeding of Twelfth International Waste Management and Landfill. Sardinia 2009 Symposium, 2009, Sardinia, Italy.

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Abstract

Accurate enumeration of Salmonella spp. is important for assessing whether this pathogen has survived composting. Recent literature has reported that enumeration of Salmonella spp. using standard microbiological methods has a numbers of disadvantages, particularly the time taken to obtain a result. This research is an attempt to develop a rapid, low-cost detection method that is quantitative, highly sensitive and target specific. This paper reports the development of a DNA fragment that can be used to quantify Salmonella spp. by competitive polymerase chain reaction (cPCR) targeted at the invA gene of Salmonella (PCR primers that target the invA gene are reported to have very high specificity for Salmonella strains). It is shown that cPCR, which could be completed in 5 hours, could quantify the number of copies of the Salmonella invA gene in a sample solution.

Item Type: Conference or Workshop Item (Paper)
Subjects: Q Science > QR Microbiology
T Technology > TD Environmental technology. Sanitary engineering
Divisions: Faculty of Engineering Technology > Department of Chemical Engineering Technology
Depositing User: Dr Norshuhaila Mohamed Sunar
Date Deposited: 27 Apr 2014 03:17
Last Modified: 27 Apr 2014 03:20
URI: http://eprints.uthm.edu.my/id/eprint/5335
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