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Degradation of phenanthrene by corynebacterium urealyticum in liquid culture and sand slurry

Mohd Kamil, Nor Amani Filzah and Alias, Salina and Othman, Norzila and Abdul-Talib, Suhaimi (2013) Degradation of phenanthrene by corynebacterium urealyticum in liquid culture and sand slurry. Malaysian Journal of Soil Science, 17 (1). pp. 111-126. ISSN 1394-7990

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Abstract

Most studies on biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) evaluate the degradation potential of indigenous microorganisms in either liquid or solid media. There are limited studies on evaluation of the same microorganisms in degrading PAHs under non-indigenous condition in both liquid or solid media. This study investigated the potential of the bacteria, Corynebacterium urealyticum isolated from municipal sludge in degrading phenanthrene in both liquid and solid media. The study also evaluated the performance of the strain when subjected to low and high initial concentration of PAHs. Batch experiments were conducted over 20 days in reactors containing artificially contaminated phenanthrene minimal media and sand slurry inoculated with a bacterial culture. Phenanthrene degradation in liquid culture and sand slurry were found to be 82.15% and 27.71%, respectively. The degradation activity of bacteria in liquid culture remained active throughout the duration of the experiment, but this was not the case in the sand slurry. A significant difference was observed in the amount of phenanthrene remaining in the sand slurry when the bacteria was inoculated into the low and high phenanthrene concentrations. Percentages of phenanthrene remaining for both initial concentrations in liquid culture were not significant. From the bacteria growth curve plotted through viable count analysis, it was observed that the bacteria could immediately adapt to PAH-contaminated sand and had better capability to degrade phenanthrene in liquid culture compared to sand slurry.

Item Type: Article
Uncontrolled Keywords: biodegradation; corynebacterium urealyticum; liquid cultures; phenanthrene; sand slurry
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Civil and Environmental Engineering > Department of Water and Environmental Engineering
Depositing User: Normajihan Abd. Rahman
Date Deposited: 03 Nov 2014 06:36
Last Modified: 03 Nov 2014 06:36
URI: http://eprints.uthm.edu.my/id/eprint/5839
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