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Bioremediation of biofuel-soil contamination by using pseudomonas putida

Mohamed Sunar, Norshuhaila and Emparan, Q. and Abdul Karim, Ahmad Tarmizi and Mohd Noor, Siti Fatimah and Maslan, Masayu and Mustafa, Fazlyana and Khaled, Nadiah (2014) Bioremediation of biofuel-soil contamination by using pseudomonas putida. Advanced Materials Research, 845 (2014). pp. 138-145. ISSN 1022-6680


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The commercialization of biodiesel/diesel blends on the market can cause environmental damages due to spills. This study aims to investigate the effectiveness of Pseudomonas putida as oil-biodegradable agent in soil contaminated with biodiesel/diesel blend (B20). The effectiveness on bioremediation have been conducted by examined several physico-chemical tests on biodiesel/diesel-contaminated soil before and after seeding of P. putida. The spillage stimulation of B20 was conducted at laboratory scale for 24 days of incubation time. The results show that the bioremediation treatment able to remove up to 82%, 77%, 16%, and 10% of nitrogen, phosphate, sulfate and total organic carbon, respectively. The pH of soil sample was changed from pH 7.45 (Day 0) to 7.25 (Day 24) after the treatment. Meanwhile, the moisture content in the sample has increased from 44.11% (Day 0) to 50.35% (Day 24). All of these results show the good indication of quality improvement of polluted soil after treated with P. putida. It is apparent from the acquired results that the application of P. putida is suitable as effective microorganism and potentially exploits as useful oil-soil biodegradable agent in polluted soil.

Item Type: Article
Uncontrolled Keywords: biofuel; bioremediation; physico-chemical removal; pseudomonas putida
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA703-711 Engineering geology. Rock mechanics. Soil mechanics.
Divisions: Faculty of Engineering Technology > Department of Chemical Engineering Technology
Depositing User: Normajihan Abd. Rahman
Date Deposited: 04 Jan 2015 08:33
Last Modified: 04 Jan 2015 08:33
URI: http://eprints.uthm.edu.my/id/eprint/6188
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