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Investigation of traction patterns and forces of single quiescent and migratory keratinocytes using a novel cell traction force microscopy technique

Soon, Chin Fhong and Morgan, Denyer and Nayan, Nafarizal and Saim, Hashim (2012) Investigation of traction patterns and forces of single quiescent and migratory keratinocytes using a novel cell traction force microscopy technique. Other thesis, Universiti Tun Hussein Onn Malaysia.

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Abstract

Keratinocyte traction forces play a crucial role in wound healing. The aim of this study was to develop a novel cell traction force (CTF) transducer system based on cholesteryl ester liquid crystals (LC). The CTF microscopy system is used to analyse the traction force patterns of quiescent and migratory keratinocytes cultured on LC. We report the application of the WSPR imaging system in the study of the interaction between keratinocytes and liquid crystals (LC). Imaging of fixed keratinocytes cultured on gold coated surface plasmon substrates functionalized with a thin film of liquid crystals was performed in air using a 1.45 NA objective based system. Characteristics of the cell integrin expressions and presence of extracellular matrix (ECM) proteins on the liquid crystals were interrogated using various immunocytochemical techniques, Atomic Force Microscopy (AFM), fourier transform infrared spectroscopy (FTIR). The generated CTF map highlighted distinct distributions and different magnitude of CTFs were revealed for polarized and non-polarized keratinocytes. WSPR imaging indicated that keratinocytes are less spread and formed distinct topography of cell-liquid crystal couplings when cultured on liquid crystal coated substrates. The WSPR microcopy reveals that the cells remodeled their topography of adhesion at different interfaces. The immunostainings indicated that cell adhesion on the LC is mediated by self-secreted ECM proteins. As revealed by the AFM imaging, the constraint in cell membrane spread on the LC leads to the increase in cell surface roughness and thickness of cell membrane. The biophysical expressions of cells on biocompatible CELC suggested that CELC could be a new class of biological relevant material.

Item Type: Thesis (Other)
Subjects: Q Science > QD Chemistry
Depositing User: Normajihan Abd. Rahman
Date Deposited: 11 May 2015 08:38
Last Modified: 11 May 2015 08:38
URI: http://eprints.uthm.edu.my/id/eprint/6909
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