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The study on factors affecting the survival of eschehchu coli in dredged marine soils

Anuar, Nurasiah Mira (2015) The study on factors affecting the survival of eschehchu coli in dredged marine soils. Masters thesis, Universiti Tun Hussein Onn Malaysia.


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Dredging work involves a range of marine soils, varying from coarse to fine, clean to contaminated. Dredging involves excavation and disposal and both processes could affect the marine environment through release of possible contaminants. Dredged marine soil (DMS) samples with different physico-chemical properties from two dredging locations were analyzed for identification of pathogenic bacteria. Identification process was a first element in the detection of biological hazards in marine soils. Due to the potential for transmission of diseases, this hazard becomes a major concern as the DMS has its own values for reuse or recycle purposes. To prevent the needless cast and the involved in assessing several of pathogenic bacteria, the indicator bacteria, Escherichia coli (E.coli) has been used to assess the level of biological contamination in marine environment. The effect of natural factors including salinity, pH, marine soil particle size, solar exposure and depth of soil were investigated. The main goal is to understand the bacterial survival ability, as an approach to deal with the hazards. Under the condition without the existence of predatory microorganisms, experiments is performed at nine salinity level; ranging from 0 % to 35 %, pH 6 to 9 and nine hours of solar exposure. It was observed that salinity had induced pH changes. The highest survival was observed at higher salinity after 42 days of exposure. Besides salinity, pH also affects the survival. The pH experimental revealed the high survival of E. coli in high pH. High number of E. coli was found in clay rather than silt soil indicated the survival of bacteria was affected by the particle size as well. In laboratory solar exposure experiments, the data dealing with the survival of bacteria showed that the cell reduction was more pronounced when compared to the absence of solar threat. After an extended period of solar exposures, 2-logs of E, colt have been removed. Therefore, based on the result, the bacteria survivability in DMS was affected by the particle size, salinity, pH and solar exposures.

Item Type: Thesis (Masters)
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA703-711 Engineering geology. Rock mechanics. Soil mechanics.
Depositing User: Normajihan Abd. Rahman
Date Deposited: 30 May 2016 08:21
Last Modified: 30 May 2016 08:21
URI: http://eprints.uthm.edu.my/id/eprint/8025
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