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Effects of the immobilization of recombinant Escherichia coli on cyclodextrin glucanotransferase (CGTase) excretion and cell viability

Che Man, Rohaida and Ismail, Ahmad Fauzi and Ghazali, Nazlee Faisal and Mohd Fuzi, Siti Fatimah Zaharah and Md Illias, Rosli (2015) Effects of the immobilization of recombinant Escherichia coli on cyclodextrin glucanotransferase (CGTase) excretion and cell viability. Biochemical Engineering Journal, 98. pp. 91-98. ISSN 1369-703X

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Abstract

The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification andmorefavorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes when using Escherichia coli as a host. In this study, the effects of different polymer of hollow fiber membrane and culture conditions on the enzyme excretion, cell lysis and plasmid stability of immobilized E. coli were investigated. The cells immobilized on a hollow fiber membrane composed of a polyvinylidene fluoride (PVDF) polymer exhibited a 2–4-fold increase in CGTase excretion, over a 100% increase in plasmid stability and 28-60% reduction in cell lysis compared with free cells. Hence, the immobilization of E. coli using a hollow fiber membrane was demonstrated to increase enzyme excretion and cell stability.

Item Type: Article
Uncontrolled Keywords: immobilized cells; free cells; enzyme activity; cell lysis; adsorption; hollow fibers
Subjects: Q Science > QP Physiology
Divisions: Faculty of Applied Science and Technology > Department of Technolgy and Heritage
Depositing User: Normajihan Abd. Rahman
Date Deposited: 10 Jan 2017 07:12
Last Modified: 10 Jan 2017 07:12
URI: http://eprints.uthm.edu.my/id/eprint/8093
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