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Immobilization of horseradish peroxidase onto polyaniline nanoparticle monolayer

Othman, Siti Amira and Radiman, Shahidan and Khoo, Kok Siong and A. Rahman, Irman (2010) Immobilization of horseradish peroxidase onto polyaniline nanoparticle monolayer. World Applied Sciences Journal, 9. pp. 23-25. ISSN 18184952

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Abstract

Enzyme have been covalently bonded to a wide variety of water- imoluble polymeric supports. Recently, the class of organic polymers called ' conductive polymers' has attracted considerable attention and has been used as supports for enzyme immobilization. Among these polyaniline emeraldine base (PANI-EB) occupies an important place because of its unique propehes, including good environmental stability, the simplicity of doping process and its excellent redox recyclability enabling polymers with significant differences to be constructed by means of simple acidic or basic treatments. This study intends to see the effect and properties Horseradsh Peroxidase (HRP) when it is deposited onto PANI-EB nanoparticle monolayer by using electrodeposition techmque. During electrodeposition, HRF will be deposited on PAM-EB monolayer that act as a cathode. For UV- visible spectnun the PAN-EB with and without HRF shows two sharp absorption peaks at -3201x11 and -800nm. HRF helps in increasing the conductivity of PANI-EB. Thls support by a resistivity value obtained from four point probe measurement. Uniformity during electrodeposition can be seen from VPSEM result. As a conclusion, immobilization of enzyme onto PANI-EB can helps in increasing the conductivity properties of PANI-EB.

Item Type: Article
Uncontrolled Keywords: HRP; PANI-EB; enzyme; polymer; conductivity
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Applied Science and Technology > Department of Science
Depositing User: Normajihan Abd. Rahman
Date Deposited: 15 Aug 2016 09:07
Last Modified: 15 Aug 2016 09:07
URI: http://eprints.uthm.edu.my/id/eprint/8310
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