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Isolation and identification of concrete environment bacteria

Juki, Mohd Irwan and Anneza, L. H. and Othman, Norzila and Husnul, T. and Alshalif, A. F. (2015) Isolation and identification of concrete environment bacteria. In: Soft Soil Engineering International Conference 2015 (SEIC2015), 27-29 Oktober 2015, Langkawi, Malaysia.

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Abstract

This paper presents the isolation and molecular method for bacteria identification through PCR and DNA sequencing. Identification of the bacteria species is required in order to fully utilize the bacterium capability for precipitation of calcium carbonate in concrete. This process is to enable the addition of suitable catalyst according to the bacterium enzymatic pathway that is known through the bacteria species used. The objective of this study is to isolate, enriched and identify the bacteria species. The bacteria in this study was isolated from fresh urine and acid mine drainage water, Kota Tinggi, Johor. Enrichment of the isolated bacteria was conducted to ensure the bacteria survivability in concrete. The identification of bacteria species was done through polymerase chain reaction (PCR) and rRDNA sequencing. The isolation and enrichment of the bacteria was done successfully. Whereas, the results for bacteria identification showed that the isolated bacteria strains are Bacillus sp and Enterococus faecalis.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: bioconcrete; effective microorganism; enrichment; 16S rDNA gene sequencing
Subjects: T Technology > T Technology (General)
Divisions: Faculty of Civil and Environmental Engineering > Department of Structural and Material Engineering
Depositing User: Normajihan Abd. Rahman
Date Deposited: 13 Aug 2018 03:38
Last Modified: 13 Aug 2018 03:38
URI: http://eprints.uthm.edu.my/id/eprint/8316
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