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Effect of transforming growth factor-b3 on mono and multilayer chondrocytes

Sefat, Farshid and Youseffi, Mansour and Khaghani, Seyed Ali and Soon , Chin Fhong and Javid, Farideh (2016) Effect of transforming growth factor-b3 on mono and multilayer chondrocytes. Cytokine, 83. pp. 118-126. ISSN 10434666

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Abstract

Articular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. Transforming growth factor-beta (TGF-b), a cytokine superfamily, regulates cell function, including differentiation and proliferation. Although the function of the TGF-bs in various cell types has been investigated, their function in cartilage repair is as yet not fully understood. The effect of TGF-b3 in biological regulation of primary chondrocyte was investigated in this work. TGF-b3 provided fibroblastic morphology to chondrocytes and therefore overall reduction in cell proliferation was observed. The length of the cells supplemented with TGF-b3 were larger than the cells without TGF-b3 treatment. This was caused by the fibroblast like cells (dedifferentiated chondrocytes) which occupied larger areas compared to cells without TGF-b3 addition. The healing process of the model wound closure assay of chondrocyte multilayer was slowed down by TGF-b3, and this cytokine negatively affected the strength of chondrocyte adhesion to the cell culture surface.

Item Type: Article
Uncontrolled Keywords: Primary chondrocyte multilayer; TGF-b3; cell length; cell proliferation; model wound closure assay
Subjects: Q Science > QP Physiology
Divisions: Faculty of Electrical and Electronic Engineering > Department of Electronic Engineering
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 13 Aug 2018 03:37
Last Modified: 13 Aug 2018 03:37
URI: http://eprints.uthm.edu.my/id/eprint/8617
Statistic Details: View Download Statistic

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