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Supercritical fluid CO2 technique for destruction of pathogenic fungal spores in solid clinical wastes

Noman, Efaq A. and N. A. Rahman, Nik N. and Shahadat, Mohammad and Hideyuki , Nagao and M. Al-Karkhi, Abbas F. and Algheethi, Adel and T. Lah, Tengku N. and M. Omar, Abdul K. (2016) Supercritical fluid CO2 technique for destruction of pathogenic fungal spores in solid clinical wastes. Clean – Soil, Air, Water, 44 (12). pp. 1700-1708. ISSN 18630669

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Abstract

The present study deals with the inactivation of fungal spores in clinical waste using 5 the advanced supercritical carbon-dioxide (SC-CO2) treatment technique. The process of 6 inactivation was investigated under different conditions of pressure, temperature, and 7 processing time. The efficiency of SC-CO2 was determined in terms of log reduction and 8 inactivation rate of fungal spores. Almost 100% inactivation of initial fungal spores was 9 obtained. Predicted and experimental log reduction of fungal spores was found to be 10 5.93 and 6.00, respectively, under optimal conditions (35 MPa, 75°C in 90 min). SC-CO2 11 treated Aspergillus niger and Penicillium simplicissimum spores were examined using 12 scanning electron microscopy which showed spore damage in the form of rupture, tear, 13 and shape distortion. These damages confirmed complete spore inactivation when no 14 renewed fungal growth occurred after the treated samples were cultured onto fresh 15 media. The inactivation of fungal spores using SC-CO2 and autoclave inhibits the 16 production of lipase, cellulase, amylase, and protease enzymes. Thus, without addition 17 of any chemicals, the SC-CO2 sterilization method can be potentially used for the 18 inactivation of microorganisms in clinical waste as well as in food and pharmaceutical 19 products.

Item Type: Article
Uncontrolled Keywords: Inactivation rate; microorganisms; pathogens; sterilization method
Subjects: T Technology > TD Environmental technology. Sanitary engineering > TD785-812 Municipal refuse. Solid wastes
Divisions: Faculty of Civil and Environmental Engineering > Department of Water and Environmental Engineering
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 13 Aug 2018 03:25
Last Modified: 13 Aug 2018 03:25
URI: http://eprints.uthm.edu.my/id/eprint/8730
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