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Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the Jakun community in Johor

Sabran, Siti Fatimah (2016) Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the Jakun community in Johor. PhD thesis, Universiti Tun Hussein Onn Malaysia.

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Abstract

Tuberculosis (TB) is a major threat to human health due to its increasing global epidemiology and emerging drug-resistant strains. While traditional knowledge (TK) could be a tool for development of novel TB treatment, TK itself is at verge of loss due to modernization and deforestration. The objectives of the study were to document the TK of medicinal plants used for the treatment of TB and its related symptoms as practiced by the Jakun community of Kampung Peta, situated in Taman Negara Johor Endau Rompin; to investigate the antimycobacterial activity and mechanism of action of the selected medicinal plants; and to profile their major phytochemical constituents. Documentation of TK were analyzed qualitatively from semi-structured interviews. Among 23 species documented, water and organic crude extracts of selected plants were evaluated for their antimycobacterial activity using agar disk diffusion, resazurin microplate assay, and agar plate assay against Mycobacterium smegmatis. Upon treatment with the active crude extracts, mechanism of action was investigated via time-kill analysis, leakage of compounds absorbing at 280 nm, field emission-scanning electron microscopy, and 2D proteomic analysis. Findings showed that ethyl acetate extract of Thottea grandiflora displayed the largest inhibition zone (DIZ= 14.92 +/- 0.86 mm). Hexane extracts of Dipterocarpus sublamellatus and Tetracera macrophylla showed the lowest minimum inhibition concentration (MIC= 0.78 mg/mL). Ethyl acetate extract of T. macrophylla, and both hexane extracts of D. sublamellatus and T. macrophylla showed the lowest minimum bactericidal concentration (MBC= 3.13 mg/mL). At 4-fold of MIC, ethyl acetate extract of T. macrophylla killed the bacterial cell within 8 hours of treatment by multitarget mechanisms such as inhibition of protein and cell wall synthesis and disruption of metabolic processes. Using gas chromatography- mass spectrometry (GC-MS) analysis, pyrazole and phenanthroline derivatives were detected as among the major constituents that potentially contribute to the antimycobacterial activity. The results scientifically validated the plants used in Jakun's traditional medicine displayed promising therapeutic properties and further studies in this direction could lead to the discovery of multitargets antimycobacterial agents.

Item Type: Thesis (PhD)
Subjects: R Medicine > RS Pharmacy and materia medica
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 19 Sep 2017 02:39
Last Modified: 19 Sep 2017 02:39
URI: http://eprints.uthm.edu.my/id/eprint/9314
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