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Manipulation and immobilization of a single fluorescence nanosensor for selective injection into cells

Hashim, Hairulazwan and Hisataka, Maruyama and Taisuke, Masuda and Fumihito, Arai (2016) Manipulation and immobilization of a single fluorescence nanosensor for selective injection into cells. Sensors, 16 (2041). pp. 1-10. ISSN 14248220

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Official URL: doi:10.3390/s16122041


Manipulation and injection of single nanosensors with high cell viability is an emerging field in cell analysis. We propose a new method using fluorescence nanosensors with a glass nanoprobe and optical control of the zeta potential. The nanosensor is fabricated by encapsulating a fluorescence polystyrene nanobead into a lipid layer with 1,3,3-trimethylindolino-60-nitrobenzopyrylospiran (SP), which is a photochromic material. The nanobead contains iron oxide nanoparticles and a temperature-sensitive fluorescent dye, Rhodamine B. The zeta potential of the nanosensor switches between negative and positive by photo-isomerization of SP with ultraviolet irradiation. The positively-charged nanosensor easily adheres to a negatively-charged glass nanoprobe, is transported to a target cell, and then adheres to the negatively-charged cell membrane. The nanosensor is then injected into the cytoplasm by heating with a near-infrared (NIR) laser. As a demonstration, a single 750 nm nanosensor was picked-up using a glass nanoprobe with optical control of the zeta potential. Then, the nanosensor was transported and immobilized onto a target cell membrane. Finally, it was injected into the cytoplasm using a NIR laser. The success rates of pick-up and cell immobilization of the nanosensor were 75% and 64%, respectively. Cell injection and cell survival rates were 80% and 100%, respectively.

Item Type: Article
Uncontrolled Keywords: Nanosensor; fluorescence sensor; photochromism; zeta potential; cell injection
Subjects: T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800-8360 Electronics
Divisions: Faculty of Engineering Technology > Department of Electrical Engineering Technology
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 13 Aug 2018 03:23
Last Modified: 13 Aug 2018 03:23
URI: http://eprints.uthm.edu.my/id/eprint/9539
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