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High resolution imaging of bio-molecular binding studies using a widefield surface plasmon microscope

Abdul Jamil, M. Mahadi and Youseffi, M. and Twigg, P. C. and Britland, S. T. and Liu, S. and See, C. W. and Zhang, J. and Somekh, M. G. and Denyer, M. C. T. (2008) High resolution imaging of bio-molecular binding studies using a widefield surface plasmon microscope. Sensors and Actuators B, 129. pp. 566-574. ISSN 09254005

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Surface plasmon microscopes are mostly built around the prism based Kretschmann configuration. In these systems, an image of a sample can be obtained in terms of an intensity map, where the intensity of the image is dependent on the coupling of the light into the surface plasmons. Unfortunately the lateral resolution of these systems relies on the ability of plasmons to propagate along the metallised layer and is usually limited to a few microns unless special measures are taken. The widefield surface plasmon microscope (WSPR), used here enables surface plasmon imaging at significantly higher lateral resolutions than prism based systems. In this study we demonstrate the functionality of the WSPR by imaging a sequence of binding events between micro-patterned extracellular matrix proteins and their specific antibodies. Using the WSPR system a change in contrast was observed with each binding event. Images produced via the WSPR system were analyzed and compared qualitatively and quantitatively. Consequently, we confirm that the WSPR microscope described here can be used to study sequential monomolecular layer binding events on a micron scale. These results have significant implications in the development of new micron scale bioassays.

Item Type: Article
Uncontrolled Keywords: bio-molecular interaction; micro-contact printing; surface plasmons; high resolution
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Electrical and Electronic Engineering > Department of Electronic Engineering
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 24 Dec 2017 13:03
Last Modified: 24 Dec 2017 13:03
URI: http://eprints.uthm.edu.my/id/eprint/9630
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