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Phytochemicals, antioxidant and antiproliferative properties of five moss species from sabah, malaysia.

Abdul Karim, Fifilyana and Suleiman, Monica and Rahmat, Asmah and Abu Bakar, Mohd Fadzelly Phytochemicals, antioxidant and antiproliferative properties of five moss species from sabah, malaysia. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (10). pp. 292-297. ISSN 0975-1491

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Abstract

Objective: This study was conducted to investigate the phytochemical contents, the antioxidant and antiproliferative properties of 80% methanol, ethanol and aqueous extracts of Sphagnum cuspidatum subsp. subrecurvum, Sphagnum cuspidatulum, Sphagnum junghuhniannum, Pogonatum cirratum subsp. fuscatum and Pogonatum cirratum subsp. macrophyllum. Methods: The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. The antioxidant properties were evaluated by three different assays, namely, ferric reducing/antioxidant power (FRAP), ABTS and DPPH free radical scavenging assays. MTT assay was used to study the antiproliferative properties against selected cancer cell lines. Results: The results showed that the aqueous and ethanol extracts of S. cuspidatum subsp. Subrecurvum has the highest total phenolic and total flavonoid content with the values of 5.42 ± 1.95 mg gallic acid equivalent per g of dry sample and 2.12 ± 0.02 mg catechin equivalent per g of dry sample, respectively. The 80% methanol extracts of S. junghuhnianum has the lowest total phenolic and total flavonoid content with the values of 0.80 ± 0.12 mg gallic acid equivalent per g of dry sample and 0.03 ± 0.02 mg catechin equivalent per g of dry sample, respectively. None of the extracts displayed IC₅₀ value (concentration that inhibits 50% of free radical) at concentration tested. The 80% methanol extracts of P. cirratum subsp. Fuscatum induced antiproliferative activity against CaOV₃ (ovarian carcinoma) cell line whereas S. cuspidatulum induced antiproliferative activity against HepG2 (liver cancer) cell line.None of the extracts induce antiproliferative activity against MDA-MB-231 (non-hormone dependent breast cancer) cell line. Conclusion: Results obtained indicated that the selected mosses contained considerable amount of phenolics and flavonoids which contribute to antioxidant properties. P. cirratum subsp. Fuscastum and S. cuspidatulum have the potential to be used in pharmaceutical industry due to the ability of these two species to induce antiproliferative activity against CaOV₃ and HepG2 cancer cell lines.

Item Type: Article
Uncontrolled Keywords: Phytochemical contents; Antioxidant activity; Antiproliferative activity; Mosses.
Subjects: Q Science > QK Botany
Divisions: Faculty of Applied Science and Technology > Department of Technolgy and Heritage
Depositing User: Normajihan Abd. Rahman
Date Deposited: 08 Aug 2018 07:47
Last Modified: 08 Aug 2018 07:47
URI: http://eprints.uthm.edu.my/id/eprint/9783
Statistic Details: View Download Statistic

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