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A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1

Noor, Yusuf Muhammad and Samsulrizal, Nurul Hidayah and Jema'onc, Noor Azah Jema'onc and Lowc, Kheng Oon and Ramli, Aizi Nor Mazila and Alias, Noor Izawati and Damis, Siti Intan Rosdianah and Mohd Fuzi, Siti Fatimah Zaharah and Mat Isa, Mohd Noor and Abdul Murad, Abdul Munir and Mohd Raih, Mohd Firdaus and Abu Bakar, Farah Diba and Najimudin, Nazalan and Mahadi, Nor Muhammad and Md. Illias, Rosli A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1. Gene, 545. pp. 253-261. ISSN 0378-1119

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Abstract

Bacillus lehensis G1 is a Gram-positive,moderately alkalitolerant bacteriumisolated fromsoil samples. B. lehensis produces cyclodextrin glucanotransferase (CGTase), an enzyme that has enabled the extensive use of cyclodextrin in foodstuffs, chemicals, and pharmaceuticals. The genome sequence of B. lehensis G1 consists of a single circular 3.99 Mb chromosome containing 4017 protein-coding sequences (CDSs), of which 2818 (70.15%) have assigned biological roles, 936 (23.30%) have conserved domains with unknown functions, and 263 (6.55%) have no match with any protein database. Bacillus clausii KSM-K16 was established as the closest relative to B. lehensis G1 based on gene content similarity and 16S rRNA phylogenetic analysis. A total of 2820 proteins from B. lehensis G1 were found to have orthologues in B. clausii, including sodium–proton antiporters, transport proteins, and proteins involved in ATP synthesis. A comparative analysis of these proteins and those in B. clausii and other alkaliphilic Bacillus species was carried out to investigate their contributions towards the alkalitolerance of the microorganism. The similarities and differences in alkalitolerance-related genes among alkalitolerant/alkaliphilic Bacillus species highlight the complex mechanism of pH homeostasis. The B. lehensis G1 genome was also mined for proteins and enzymes with potential viability for industrial and commercial purposes.

Item Type: Article
Uncontrolled Keywords: Alkaliphile; Alkalitolerant; Bacillus lehensis; Whole-genome sequencing; Biotechnological potential
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Applied Science and Technology > Department of Humanities
Depositing User: Normajihan Abd. Rahman
Date Deposited: 28 Feb 2018 06:18
Last Modified: 28 Feb 2018 06:18
URI: http://eprints.uthm.edu.my/id/eprint/9789
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