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Investigation a critical selection of pulse duration effect on growth rate of HeLa cells

Milad Zaltum, Mohamed A. and Adon, Mohamad Nazib and Hamdan, Sallehuddin and Dalimin, M. Noh and Abdul Jamil , Muhammad Mahadi (2015) Investigation a critical selection of pulse duration effect on growth rate of HeLa cells. In: 2015 International Conference on BioSignal Analysis, Processing and Systems (ICBAPS), 26 - 28 May 2015, Kuala Lumpur, Malaysia.

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Abstract

Electroporation is a process of the biophysical effect on cells exposed to an external electrical field is gaining applications in medical treatments, especially to create pores through a cell membrane and allow uptake of DNA into a cell. Therefore, in the theoretical evaluation of electroporation, transmembrane potential and characteristics of the cells growth is the target of the analysis. In this study, we measured the relation between amplitude and duration, used cervical cancer cells (HeLa cells) to be sample for electroporation, because HeLa cells is one of the most well-known cell lines, and easy to researched by continuous harvesting of large numbers of HeLa cells for in-vitro experimental tests. Cells were submitted to single pulses, at constant field strength of 2kV/cm, at various pulse durations from 30μs to 600μs. As a summary, it was found pulse duration equal to100μs is associated the HeLa cell growth rate faster confluence in comparison to the cell with 30μs, 300μs and 600μs pulse duration.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Electroporation; microsecond pulsed electric field (μsPEF); pulse duration; HeLa cell
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Electrical and Electronic Engineering > Department of Electronic Engineering
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 13 Aug 2018 03:20
Last Modified: 13 Aug 2018 03:20
URI: http://eprints.uthm.edu.my/id/eprint/9842
Statistic Details: View Download Statistic

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