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Investigation of electroporation effect on HT29 cell lines adhesion properties

Mamman, Hassan Buhari and Abdul Jamil , Muhammad Mahadi (2015) Investigation of electroporation effect on HT29 cell lines adhesion properties. In: 2015 2nd International Conference on Biomedical Engineering (ICoBE), 30-31 March 2015, Penang, Malaysia.

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Abstract

Electroporation is a method of permeabilizing a living cell membrane with high electric field intensity of short duration. This study investigates the effect of electroporation on colon cell line, HT29 adhesion Property as a preliminary study of the desire to design a potential application of electroporation on wound healing. The study found out that when HT29 cell lines were electroporated with 600 V/cm and 500 μs pulse duration it attached to the monolayer after 12 minutes of Electroporation, and covers a distance of 38.7 μm after one hour. While the non-electroporated cell line for control under the same condition attached to monolayer after 37minutes from floating state and covers a distance of 31.53 μm after 55 minutes. The result showed that electroporated HT29 cell line can attached faster and spread wider than non-electroporated HT29 cell line that can be used to facilitate cell migration for wound healing application.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Electroporation; HT29; sterilization; adhesion; migration
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Electrical and Electronic Engineering > Department of Electronic Engineering
Depositing User: Mr. Mohammad Shaifulrip Ithnin
Date Deposited: 13 Aug 2018 03:20
Last Modified: 13 Aug 2018 03:20
URI: http://eprints.uthm.edu.my/id/eprint/9844
Statistic Details: View Download Statistic

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