In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique

Leong, Wai Yean and Soon , Chin Fhong and Wong, Soon Chuan and Tee , Kian Sek and Cheong, Sok Ching and Gan, Siew Hua and Youseffi, Mansour (2017) In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique. Bioengineering, 4 (2). pp. 1-14. ISSN 23065354

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Official URL: doi:10.3390/bioengineering4020043

Abstract

Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 μL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 μm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies.

Item Type:Article
Uncontrolled Keywords:Alginate; aerosol; microencapsulation; microtissues; keratinocytes; oral squamous cell carcinoma
Subjects:R Medicine > R Medicine (General)
Divisions:Faculty of Electrical and Electronic Engineering > Department of Electronic Engineering
ID Code:10301
Deposited By:Mr. Mohammad Shaifulrip Ithnin
Deposited On:15 Aug 2018 15:25
Last Modified:15 Aug 2018 15:25

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