Induction of Apoptosis and Modulation of Caspase Activity on MCF-7 Human Breast Cancer Cells by Bioactive Fractionated Cocoa Leaf Extract

Yazan Ranneh, Yazan Ranneh and Abu Bakar, Mohd. Fadzelly and Abdah Md Akim, Abdah Md Akim and Baharum, Zainal and Mohammed S Ellulu, Mohammed S Ellulu and Abdulmannan Fadel, Abdulmannan Fadel (2023) Induction of Apoptosis and Modulation of Caspase Activity on MCF-7 Human Breast Cancer Cells by Bioactive Fractionated Cocoa Leaf Extract. Anti-Breast Cancer Activities of Cocoa, 24 (7). pp. 2473-2483.

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Official URL: https://doi.org/10.31557/APJCP.2023.24.7.2473

Abstract

Background: The objective of this study was to investigate the potential anti-proliferative activities of a methanolic extract of cocoa leaves (CL) obtained through sequential partition and fractionation against MCF-7 breast cancer cells. Methods: The methanolic extract of CL was partitioned in three separated solvents (hexane, dichloromethane, and methanol). Hexane partition was the most potent against MCF-7 cells growth with the lowest IC50 value. Then, it was subjected to two fractionation procedures, resulting in the identification of the CL bioactive fraction (II-F7) with potent toxicity against MCF-7 cells. Results: Further investigation into CL bioactive fraction (II-F7) revealed significant dose-dependent growth inhibitory effects on MCF-7 cells, which were attributed to the induction of apoptosis, as evidenced by the presence of apoptotic bodies, fragmented DNA, and disruption of mitochondrial membrane potential. Additionally, treatment with CL bioactive fraction (II-F7) upregulated the expression of pro-apoptotic genes (DDIT3, GADD45G and HRK) and significantly increased the activities of caspase-8 and caspase-9. Conclusion: Overall, this study suggests that bioactive fraction (II-F7) from CL extract has significant and selective cytotoxicity against MCF-7 cells through inducing apoptosis and has potential as a therapeutic agent for breast cancer treatment.

Item Type:	Article
Uncontrolled Keywords:	breast cancer- cocoa leaf- bioactive- fractionation- apoptosis- gene expression- MCF-7
Subjects:	T Technology > T Technology (General)
Depositing User:	Mr. Mohamad Zulkhibri Rahmad
Date Deposited:	15 Jan 2024 07:29
Last Modified:	15 Jan 2024 07:29
URI:	http://eprints.uthm.edu.my/id/eprint/10583

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