Interfacial study of cell adhesion to liquid crystals using widefield surface plasmon resonance microscopy

Soon , Chin Fhong and Khaghani, Seyed Ali and Youseffi, Mansour and Nayan, Nafarizal and Saim, Hashim and Stephen, Britland and Nick, Blagden and Clive, Morgan Thomas Denyer (2013) Interfacial study of cell adhesion to liquid crystals using widefield surface plasmon resonance microscopy. Colloids and Surfaces B: biointerfaces, 110 . ISSN 0927-7765

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Official URL: http://www.journals.elsevier.com/colloids-and-surf...

Abstract

Widefield surface plasmon resonance (WSPR) microscopy provides high resolution imaging of interfacial interactions. We report the application of the WSPR imaging system in the study of the interaction between keratinocytes and liquid crystals (LC). Imaging of fixed keratinocytes cultured on gold coated surface plasmon substrates functionalized with a thin film of liquid crystals was performed in air using a 1.45 NA objective based system. Focal adhesion of the cells adhered to glass and LC were further studied using immunofluorescence staining of the vinculin. The imaging system was also simulated with 2 x 2 scattering, matrix to investigate the optical reflection of the resonant plasmonic wave via the glass/gold/LC/cell layers. WSPR imaging indicated that keratinocytes are less spread and formed distinct topography of cell-liquid crystal couplings when cultured on liquid crystal coated substrates. The simulation indicates that glass/LC shifted the surface plasmon excitation angle to 75.39' as compared to glass/air interface at 44'. The WSPR microscopy reveals that the cells remodelled their topography of adhesion at different interfaces.

Item Type:Article
Uncontrolled Keywords:widefield surface plasmon resonance; microscopy; cell adhesion; keratinocytes; cell-liquid crystal interface
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Faculty of Electrical and Electronic Engineering > Department of Electronic Engineering
ID Code:3972
Deposited By:Normajihan Abd. Rahman
Deposited On:24 Jul 2013 12:25
Last Modified:24 Jul 2013 12:25

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