Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis

Noman, Efaq and Al-Gheethi, A. A. and A. Talip, Balkis and Mohamed, Radin and Kassim, Amir Hashim (2020) Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis. Journal of Hazardous Materials, 386. pp. 1-13. ISSN 0304-3894

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Abstract

The present study deals with optimizing, producing, characterizing, application and techno- economic analysis of oxidative enzymes [Laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP)] from Aspergillus iizukae EAN605 in submerged fermentation process using pumpkin peels as a production substrate. The best operating parameters for producing Lac, MnP and LiP (6.15, 2.58 and 127.99 U mg−1 respectively) were recorded with 20 g 100 mL−1 of substrate, 4.6 mL 100 mL−1 of inoculum size at pH 5.5 after 10 days. The crude enzyme exhibited high stability at pH (3–9) and temperatures (20−60 °C). Km (Michaelis–Menten) of Lac, MnP and LiP crude enzyme was 2.25, 1.79 and 0.72mM respectively. The decolourization of Remazol Brilliant Blue R by the crude enzyme was 84.84 %. The techno-economic analysis was assessed for a production unit with an annual operating time for enzymatic production and application is 7920 h/year and 100 m3 of the capacity. The process would produce 27,000 cm3 of crude enzyme with a price of USD 0.107 per cm3 compared to USD 1 per cm3 of the current commercial enzyme. The findings indicated that pumpkin peels have potential as a production substrate for oxidative enzymes from A. iizukae EAN605 and is economically feasible.

Item Type:	Article
Uncontrolled Keywords:	Submerged fermentation; Laccase; Manganese peroxidase; Lignin peroxidase; Decolourization; Response surface methodology
Subjects:	T Technology > T Technology (General) T Technology > TP Chemical technology > TP200-248 Chemicals: Manufacture, use, etc.
Divisions:	Faculty of Civil Engineering and Built Environment > Department of Civil Engineering : Water and Environmental Engineering
Depositing User:	Miss Afiqah Faiqah Mohd Hafiz
Date Deposited:	03 Jan 2022 02:56
Last Modified:	03 Jan 2022 02:56
URI:	http://eprints.uthm.edu.my/id/eprint/4988

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