Measurement and mapping of cell traction forces on liquid crystal based force transducer

Chin, Fhong Soon and Youseffi, Mansour and Nick, Blagden and Morgan, Denyer (2011) Measurement and mapping of cell traction forces on liquid crystal based force transducer. In: Proceedings of the IASTED International Conference Computational Bioscience (CompBio 2011), 11 - 13 July 2011, Cambridge, United Kingdom.

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Abstract

A novel cell traction force transducer based on shear sensitive cholesteryl ester liquid crystals (LCTFT) has been developed. This transducer incorporated with a custom-built cell traction force measurement and mapping (CTFM) software showed improved sensitivity and spatial resolution in sensing traction forces of human keratinocytes. The sensing output of the LCTFT was displayed as linear deformation lines in the liquid crystal surface that were induced by contraction of actin filaments via the focal adhesions of cells. In this context, the traction forces that were exerted locally by two clusters of focal adhesions induced strains in bi-axial directions in the liquid crystal surface. By using cell relaxation technique, these strains were determined and they were correlated by Poisson’s ratio. With the displacement information, traction forces of single cells were calculated based on Hooke’s theorem and the force-deformation relationships were derived. In this study, the transducer detected a wide range of cell traction forces (~10 - 140 nN) indicating the sensitivity of the system. The cell traction force maps generated by our CTFM software at a spatial resolution of ~ 5μm showed good representations of localised cell traction force fields and might be useful for time-based study of dynamic cells tractions.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:liquid crystals; cell traction force mapping; cell force sensor; keratinocytes; Poisson’s ratio
Subjects:Q Science > QD Chemistry
Divisions:Faculty of Electrical and Electronic Engineering > Department of Electronic Engineering
ID Code:6064
Deposited By:Normajihan Abd. Rahman
Deposited On:26 Feb 2015 14:34
Last Modified:26 Feb 2015 14:34

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