Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose

Z Abidin, Munira and Kourmentza, Constantina and K Karatzas, Andreas and Niranjan, Keshavan (2019) Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose. Journal of Chemical Technology and Biotechnology, 94 (8). pp. 2529-2536. ISSN 0268-2575

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Abstract

Background: n,n′-diacetylchitobiose (glc n ac2) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre-treatment process using steam under pressure in an autoclave was employed to facilitate subsequent enzymatic hydrolysis of chitin with chitinase from streptomyces griseus. Results: pre-treatment of chitin with 0.05mol l−1 sodium acetate buffer (p h= 6.0) at 121 ∘c for 60 min, followed by enzymatic hydrolysis involving24 hincubation,were foundtobethebest conditions for producing glc n ac2. the glc n ac2 thus obtainedwas tested regarding its antimicrobial activity against gram-negative and gram-positive strains and showed minimum inhibitory concentrations at 5 and 10% (w/v) against escherichia coli k-12 and listeria monocytogenes 10403 s, respectively. Conclusions: the extent of swelling and crystallite size of chitin increased with pre-treatment residence time, and enhanced the rate of subsequent hydrolysis using chitinase. © 2019 society of chemical industry

Item Type:	Article
Uncontrolled Keywords:	thermal pre-treatment; enzymatic hydrolysis; GlcNAc2; antimicrobial activity
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Applied Science and Technology > Department of Technology and Natural Resources
Depositing User:	Miss Afiqah Faiqah Mohd Hafiz
Date Deposited:	26 Jan 2022 08:53
Last Modified:	26 Jan 2022 08:53
URI:	http://eprints.uthm.edu.my/id/eprint/6136

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