Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans

ABDUL-MANAN, MUHAMMAD NURISMADEE and MOHD-RIDWAN, ABD RAHMAN and AIFAT, NOR RAHMAN and OSMAN, NUR AZIMAH and Abdul-Latiff, Muhammad Abu Bakar and DHARMALINGAM, SABAPATHY and MD-ZAIN, BADRUL MUNIR (2020) Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans. BIODIVERSITAS, 21 (8). pp. 3665-3669. ISSN 1412-033X

Text
AJ 2020 (450).pdf
Restricted to Registered users only
Download (245kB) | Request a copy

Official URL: https://doi.org/10.13057/biodiv/d210832

Abstract

Abdul-Manan MN, Mohd-Ridwan AR, Aifat NR, Osman NA, Abdul-Latiff MA, Dharmalingam S, Md-Zain BM. 2020. Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans. Biodiversitas 21: 3665-3669. Genetic identification of captive orangutans is of paramount importance in providing a correct identity that is essential for captive management. Thus, the utility of nuclear DNA sequences was tested in this study to identify the genetic identity of captive orangutans at Bukit Merah Orang Utan Island. Out of 24 DNA samples that were successfully extracted, only 10 orangutan samples were successfully sequenced for the von Willebrand factor (vWF) gene. From the results, this gene was able to separate the genus Pongo at the species level. Distance and character analyses indicated that a clear separation between P. pygmaeus and P. abelii at the species level. However, the degree of separation at species level was indicated in tree topology with moderate bootstrap values. At the subspecies level of P. pygmaeus, this gene was unable to show a clear separation between three Bornean subspecies. All the subspecies were formed clade together with each other. The vMF gene is a good nuclear gene for the study of phylogenetic relationships of orangutans in captivity at the species level, but the genetic identification at subspecies level in the genus level remains unclear. We suggest that future studies should involve multiple independent nuclear markers to increase the probability of getting reliable results.

Item Type:	Article
Uncontrolled Keywords:	Captive orangutan, nuclear DNA, von Willebrand Factor (vMF) gene, phylogenetic, Pongo
Subjects:	T Technology > TP Chemical technology > TP155-156 Chemical engineering
Divisions:	Faculty of Applied Science and Technology > Department of Technology and Natural Resources
Depositing User:	Mr. Shahrul Ahmad Bakri
Date Deposited:	05 Apr 2022 06:23
Last Modified:	05 Apr 2022 06:23
URI:	http://eprints.uthm.edu.my/id/eprint/6859

Actions (login required)

View Item