Alteration of the bone tissue material properties in type 1 diabetes mellitus: a fourier transform infrared microspectroscopy study

Aleksandra, Mieczkowska and Mansur, Sity Aishah and Nigel, Irwin and Peter R., Flatt and Daniel , Chappard and Guillaume , Mabilleau (2015) Alteration of the bone tissue material properties in type 1 diabetes mellitus: a fourier transform infrared microspectroscopy study. Bone, 76 . pp. 31-39. ISSN 8756-3282

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Official URL: http://dx.doi.org/10.1016/j.bone.2015.03.010

Abstract

Type 1 diabetes mellitus (T1DM) is a severe disorder characterized by hyperglycemia and hypoinsulinemia. A higher occurrence of bone fractures has been reported in T1DM, and although bone mineral density is reduced in this disorder, it is also thought that bone quality may be altered in this chronic pathology. Vibrational microscopies such as Fourier transform infrared microspectroscopy (FTIRM) represent an interesting approach to study bone quality as they allowinvestigation of the collagen andmineral compartment of the extracellularmatrix in a specific bone location. However, as spectral feature arising fromthemineral may overlapwith those of the organic component, the demineralization of bone sections should be performed for a full investigation of the organic matrix. The aims of the present study were to (i) develop a new approach, based on the demineralization of thin bone tissue section to allow a better characterization of the bone organic component by FTIRM, (ii) to validate collagen glycation and collagen integrity in bone tissue and (iii) to better understandwhat alterations of tissue material properties in newly forming bone occur in T1DM. The streptozotocin-injected mouse (150mg/kg body weight, injected at 8 weeks old) was used as T1DM model. Animals were randomly allocated to control (n = 8) or diabetic (n = 10) groups and were sacrificed 4 weeks post-STZ injection. Bones were collected at necropsy, embedded in polymethylmethacrylate and sectioned prior to examination by FTIRM. FTIRM collagen parameters were collagen maturity (area ratio between 1660 and 1690 cm−1 subbands), collagen glycation (area ratio between the 1032 cm−1 subband and amide I) and collagen integrity (area ratio between the 1338 cm−1 subband and amide II). No significant differences in the mineral compartment of the bone matrix could be observed between controls and STZ-injected animals. On the other hand, as compared with controls, STZ-injected animals presented with significant higher value for collagen maturity (17%, p = 0.0048) and collagen glycation (99%, p = 0.0121), while collagen integrity was significantly lower by 170% (p = 0.0121). This study demonstrated the profound effect of early T1DM on the organic compartment of the bone matrix in newly forming bone. Further studies in humans are required to ascertainwhether T1DMalso lead to similar effect on the quality of the bone matrix.

Item Type:Article
Uncontrolled Keywords:fourier transform infrared microspectroscopy; type 1 diabetes mellitus; collagen glycation; collagen integrity
Subjects:Q Science > QC Physics
Divisions:Faculty of Engineering Technology > Department of Chemical Engineering Technology
ID Code:8084
Deposited By:Normajihan Abd. Rahman
Deposited On:10 Jan 2017 12:27
Last Modified:10 Jan 2017 12:27

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