Antibacterial activities of lipid and pigment extract of Scenedesmus sp. isolated from the temporary waters of Endau Rompin

Ishaq, Aisha Gogoba (2016) Antibacterial activities of lipid and pigment extract of Scenedesmus sp. isolated from the temporary waters of Endau Rompin. Masters thesis, Universiti Tun Hussein Onn Malaysia.

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Abstract

The search for alternatives that could circumvent the use of synthetic antimicrobial compounds is the result of a worldwide demand for novel antimicrobial agents from natural sources. This study covered the screening and investigation of the antibacterial potential of freshwater green microalga Scenedesmus sp. isolated from temporary rock pools of Taman Negara Johor Endau Rompin, Malaysia. Crude pigment and lipid extracts from the microalga were tested using the agar well diffusion method for their antibacterial activity against foodborne pathogens, Staphylococcus aureus (ATCC 25923) and Salmonella sp. (ATCC 14028). Following that, antibacterial activities of the extracts were evaluated in food spiked with Staphylococcus aureus (ATCC 25923) through bacterial colony count. The minimum inhibitory concentrations (MIC) of both extracts were assessed using macro broth dilution assay. The results of this study showed the pigment extract at concentrations ranging from 0.35 mg/ml – 3.48 mg/ml exhibited antibacterial activity against Staphylococcus aureus (ATCC 25923) with mean zones of inhibition from 4.67 mm – 17.33 mm. Likewise, the lipid extract at concentrations ranging from 0.085 mg/ml - 0.85 mg/ml exhibited antibacterial activity against Staphylococcus aureus (ATCC 25923) with mean zones of inhibition from 1.33 mm – 15.67 mm. However, both extracts did not show any activity against Salmonella sp. (ATCC 14028). The MIC of the pigment and lipid extracts were 80 μg/ml and 21 μg/ml respectively. In food spiked with S. aureus (ATCC 25923), outnumbered bacterial colonies were observed from both samples of lipid (0.1 mg/ml - 0.2 mg/ml) and pigment (0.41 mg/ml - 0.81 mg/ml). However, there was no growth of bacterial colonies in both samples of lipid at 0.8 mg/ml - 1 mg/ml and pigment at 2.83 mg/ml - 4.05 mg/ml. The results of the antibacterial activity indicates that Scenedesmus sp. extracts of either lipid or pigment contains secondary metabolites with great potential as a food additive.

Item Type:Thesis (Masters)
Subjects:T Technology > TD Environmental technology. Sanitary engineering > TD895-899 Industrial and factory sanitation
ID Code:9065
Deposited By:Mr. Mohammad Shaifulrip Ithnin
Deposited On:17 Apr 2017 16:12
Last Modified:17 Apr 2017 16:12

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