Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration

Ong, Pauline and Jian, Jinbao and Li, Xiuhua and Yin, Jianghua and Ma, Guodong (2024) Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 305. pp. 1-15.

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Abstract

Spectroscopy in the visible and near-infrared region (Vis-NIR) region has proven to be an effective technique for quantifying the chlorophyll contents of plants, which serves as an important indicator of their photosynthetic rate and health status. However, the Vis-NIR spectroscopy analysis confronts a significant challenge concerning the existence of spectral variations and interferences induced by diverse factors. Hence, the selection of characteristic wavelengths plays a crucial role in Vis-NIR spectroscopy analysis. In this study, a novel wavelength selection approach known as the modified regression coefficient (MRC) selection method was introduced to enhance the diagnostic accuracy of chlorophyll content in sugarcane leaves. Experimental data comprising spectral reflectance measurements (220–1400 nm) were collected from sugarcane leaf samples at different growth stages, including seedling, tillering, and jointing, and the corresponding chlorophyll contents were measured. The proposed MRC method was employed to select optimal wavelengths for analysis, and subsequent partial least squares regression (PLSR) and Gaussian process regression (GPR) models were developed to establish the relationship between the selected wavelengths and the measured chlorophyll contents. In comparison to full-spectrum modelling and other commonly employed wavelength selection techniques, the proposed simplified MRC-GPR model, utilizing a subset of 291 selected wavelengths, demonstrated superior performance. The MRC-GPR model achieved higher coefficient of determination of 0.9665 and 0.8659, and lower root mean squared error of 1.7624 and 3.2029, for calibration set and prediction set, respectively. Results showed that the GPR model, a nonlinear regression approach, outperformed the PLSR model.

Item Type:	Article
Uncontrolled Keywords:	Chlorophyll Gaussian process regression Partial least squares regression Sugarcane Wavelength selection
Subjects:	S Agriculture > SB Plant culture > SB183 -317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants
Divisions:	Faculty of Mechanical and Manufacturing Engineering > FKMP
Depositing User:	Mr. Mohamad Zulkhibri Rahmad
Date Deposited:	13 Nov 2024 23:57
Last Modified:	14 Nov 2024 00:00
URI:	http://eprints.uthm.edu.my/id/eprint/11676

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