Optimizing of pharmaceutical active compounds biodegradability in secondary effluents by β-lactamase from Bacillus subtilis using central composite design

Al-Gheethi, Adel and Noman, Efaq and Radin Mohamed, Radin Maya Saphira and Ismail, Norli and Abdullah, Abd Halid and Mohd Kassim, Amir Hashim (2019) Optimizing of pharmaceutical active compounds biodegradability in secondary effluents by β-lactamase from Bacillus subtilis using central composite design. Journal of Hazardous Materials, 365. pp. 883-894. ISSN 0304-3894

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Official URL: https://doi.org/10.1016/j.jhazmat.2018.11.068

Abstract

Biodegradation of pharmaceuticals active compounds (PACs) in secondary effluents by using B. subtilis 2012WTNC as a function of β-lactamase was optimized using response surface methodology (RSM) designed by central composite design (CCD). Four factors including initial concentration of bacteria (1–6 log10 CFU mL−1), incubation period (1–14 days), incubation temperature (20–40 °C) and initial concentration of PACs (1–5mg L−1) were investigated. The optimal operating factors for biodegradation process determined using response surface methodology (RSM) was recorded with 5.57 log10 CFU mL−1 of B. subtilis, for 10.38 days, at 36.62 °C and with 4.14 mg L−1 of (cephalexin/amoxicillin) with R2 coefficient of 0.99. The biodegradation was 83.81 and 93.94% respectively. The relationship among the independent variables was significant (p < 0.05) with 95% of confidence level at the best operating parameters. The bioassay for PACs after the degradation

Item Type:	Article
Uncontrolled Keywords:	B. subtilis; Amoxicillin; Cephalexin; Bioactivity; Degradation pathway
Subjects:	T Technology > TP Chemical technology > TP200-248 Chemicals: Manufacture, use, etc.
Divisions:	Faculty of Civil Engineering and Built Environment > Department of Civil Engineering : Water and Environmental Engineering
Depositing User:	Miss Afiqah Faiqah Mohd Hafiz
Date Deposited:	25 Nov 2021 03:31
Last Modified:	25 Nov 2021 03:31
URI:	http://eprints.uthm.edu.my/id/eprint/4105

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